Neuroleptics radioreceptor assay

Javaid Jl, Pandey GN, Duslak B, et al. Measurement of neuroleptic concentrations by GLC and radioreceptor assay. Common Psychopharmacol 1980 4 467-475. 

Calil H. M., Avery D H, Hollister L. E, Creese I, and Snyder S H (1979) Serum levels of neuroleptics measured by dopamine radioreceptor assay and some clinical observations Psychiatr Res 1,39-44... 

Tune L. E, Creese I, DePaulo R., Slavney P R., Coyle J T., and Snyder S H. (1980) Clinical state and serum neuroleptic levels measured by radioreceptor assay in schizophrenia Am,] Psyc/nafr. 137,187-190... 

In order to demonstrate specificity, investigators have quantified samples using the radioreceptor assay and some other method of analysis, on the premise that if the values obtained are similar, then the radioreceptor assay is appropriate under the conditions employed. For example, the serum concentration of neuroleptics can be determined using a radioreceptor assay based on the dopamine receptor binding procedure (Creese and Snyder, 1977). To demonstrate the specificity of this assay, some samples were analyzed using both the radioreceptor assay and a radioimmunoassay (Table 3). The results obtained were virtually identical, indicating that the radioreceptor assay was measuring the same substance as that detected by radioimmunoassay. 

Radioreceptor assays have also been devised to measure blood levels of neuroleptics, p-receptor antagonists, and benzodiazepines. The development of these assays has an important clinical application since proper monitoring of blood levels is vital for maximizing therapeutic response, while minimizing toxicity. 

---