Neuroblastoma delta receptors

Both groups used expression cloning to screen cDNA libraries obtained from NG 108-15 mouse neuroblastoma-rat glioma hybridoma cells. These cells were a logical choice for delta opioid receptor cloning efforts, since they express high density of delta receptors and can be produced in great quantities in cell culture. 

Laugwitz K-L, Offermanns S, Spicher K et al. mu and delta opioid receptors differentially couple to G protein sub-types in membranes of human neuroblastoma SH-SY5Ycells. Neuron 1993 10 233-242. 

The delta opioid receptor also could stimulate the PLC activity and increase intracellular Ca2+ level in mechanism other than the activation of Gi/Go proteins. In a human neuroblastoma cell line, SK-N-BE, delta opioid receptors mobilize Ca2+ from intracellular ryanodine-sensitive stores which is independent of the PTX-sensitive G /G0 proteins [94]. Coexpressing the delta opioid receptor with G16, a promiscuous G protein, allows for a PTX-insensitive stimulation of the PLC activity by the opioid agonist [95]. Thus, the ability of the opioid receptors to stimulate PLC(3 is determined in part by the availability of complementary G proteins in any particular cell type. 

---