CDNA screening

R. Drmanac and S. Drmanac, cDNA screening by array hybridization. Meth. Enzymol. 303, 165-178 (1990). 

The cloning of the delta receptor set in motion a competitive race to identify other members of the opioid receptor family. Homologous orphan clones were quickly assessed for opioid receptor binding properties, which resulted in the identification of the kappa receptor and reassignment of an orphan clone as the delta opioid receptor [31]. PCR, genomic, and cDNA screens revealed the mu opioid receptor and an extremely abundant orphan member, named opioid receptor-like (ORL-1) receptor (reviewed by Massotte and Kieffer [30]). 

In rat aortic cells, phospholamban is expressed in differentiated freshly dispersed cells and is absent from dedifferentiated late-passage cells as shown by differential cDNA screening (Shanahan et al, 1993). 

Targets Identified in cDNA screens with Purvalanol B-MFC MFC = Methotrexate fusion compound CDK2 CMCC 3 + + + 9a ... 

Figure 5. cDNA screening. E, coli transformants grown in duplicate were hybridized to either P-labeled mRNA from low COg grown wild type cells (A) or high CO2 grown CIA-5 cells (B). 

Huang, G. M., Farkas, J., and Hood, L. (1996) High-throughput cDNA screening utilizing a low order neural network filter. BioTechniques 21, 1110-1114. 

Screening cDNA Expression Libraries with Antibodies... 

Homology screening. Using oligonucleotide probes based on known receptor sequences, search cDNA libraries for homologous sequences which may code for related receptors. The clones are then isolated and sequenced and used in expression studies to confirm the identity of the receptor. 

The PE2 isoform has been purified and fully sequenced (Markovic and Joumval 1986). Using this sequence data Ray et al (1988) succeeded in isolating a clone fi om a tomato fruit cDNA library. The predicted amino acid sequence fi om this clone had high homology to the actual amino acid sequence determined for PE2 but was not identical. Subsequent screening of the tomato... 

The amino acid sequence of the human erythrocyte glucose transporter was deduced from the nucleotide sequence of a cDNA clone in 1985 [106]. Polyclonal antibodies raised against the protein were used to screen a Xgtl I cDNA library prepared from the human hepatocellular carcinoma cell line HepG2. (Like many other transformed... 

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