Neuraminidases substrate specificities

X 10 ) and substrate specificities, and exhibited similar behaviour towards ions they differed slightly in pH optima, thermostabilities, and kinetic properties. The neuraminidases differ from Clostridium perfringens neuraminidase (mol. wt. 5.7 X 10 ) in substrate specificity, and were not inhibited by 4-chloro-mercuribenzoate. 

Baum LG, Paulson JC. (1991) The N2 neuraminidase of human influenza virus has acquired a substrate specificity complementary to the hemagglutinin receptor specificity. Virology 180, 10-15. 

Huang, R.T.C., and Orlich, M., 1972, Substrate specificities of the neuraminidases of Newcastle disease and fowl plague viruses, Hoppe-Seyler s Z. Physiol. Chem. 353 318-322. 

Saito, M., and Yu, R. K., 1986, Further characterization of a myelin-associated neuraminidase Properties and substrate specificity, J. Neurochem. 47 632-641. 

Holmquist, L., and Brossmer, R., 912a. On the specificity of neuraminidase. The carboxymethyl a-ketoside of N-acetyl-D-neuraminic acid, a Vibrio cholerae neuraminidase substrate having two anionic sites, FEBS Lett. 22 46. 

Lukert (1972) made the interesting observation, which may not, however, be directly related to substrate specificity, that the attachment of avian, infectious bronchitis virus to monolayers of chicken embryo kidney cells was inhibited not only by the addition of free or bound sialic acid, but by sulfhydryl-containing compounds. Receptors could be destroyed by neuraminidase or p-hydroxymercuribenzoate treatment. The adsorption of Newcastle disease virus, however, was not affected by the addition of sulfhydryl compounds. 

Drzeniek, R., and Gauhe, A., 1970, Differences in substrate specificity of myxovirus neuraminidases, Biochem. Biophys. Res. Commun. 38 651. 

In contrast to fliese hansmolecular TSs, the group of Yu-Teh Li described an intramoleeular TS in die leech that forms 2,7-anhydro-Neu5Ac upon release of siahc acid fiom die glycosidic linkage. Such an enzyme ( neuraminidase B ) was found in Streptococcus pneumoniae by Gut et al This enzyme has a strict specificity for a-(2 3)-linked sialic acid substrates. 

Kessler, J., Heck, J., Tannenbaum, S. W., and Flashner, M., 1982, Substrate and product specificity of Arthrobacter sialophilus neuraminidase, J. Biol. Chem. 257 5056-5060. 

Kodama, H., Baum, L. G., and Paulson, J. C., 1991, Synthesis of linkage-specific sialoside substrates for colorimetric analysis of neuraminidases, Carbohydr. Res. 218 111-119. 

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