Neoglycoproteins as Vaccines Against Haemophilus influenzae Type

Neoglycoproteins as Vaccines Against Haemophilus influenzae Type b 

A sulfur-based chemoselective method was applied to the synthesis of a conjugate polysaccharide vaccine against Haemophilus influenzae type b.37 The 

An A -methyl-amino-oxy amino acid has also been incorporated into peptides using standard SPPS procedures. Reaction of these peptides with native reducing sugars yields neoglycopeptides via a chemoselective reaction with the amino-oxy side chains, maintaining the linked sugars in their cyclic conformations and 

Neoglycosylation of proteins was also applied to the modification of the psychrophilic Atlantic cod trypsin the applicability of psychrophilic enzymes is limited because of their lower thermodynamic stability, despite their higher catalytic rate. It has been shown that the thermodynamic stability could be enhanced appreciably by covalent chemical modification with an oxidized sucrose polymer without affecting the enzymatic activity. The acquired stability of cod trypsin was found to be on par with the mesophilic porcine trypsin.40 

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