Nanocomplex

New metallo-nanostructured materials of carbon nanohorns were recently prepared by the coordination of Cu(II)-2,2 6,2,-terpyridine (Cuntpy) with oxidized carbon nanohorns (CNHs-COOH) and the resulted CNHs-COO-Cuntpy metallo-nanocomplexes have shown efficient fluorescence quenching, suggesting that electron transfer occurs from the singlet excited state of Cuntpy to CNHs.76... 

Zimet, P., Livney, Y.D. (2009). Beta-lactoglobulin and its nanocomplexes with pectin as vehicles for co-3 polyunsaturated fatty acids. Food Hydrocolloids, 23, 1120-1126. 

Fig. 10.8 Schematic representation of the formation of pectin-liposome nanocomplexes (PLNs) (taken from Sriamornsak et al. 2008)...

Sriamornsak P, Thirawong N, Nunthanid J, Puttipipatkhachorn S, Thongborisute J, Takeu-chi H (2008) Atomic force microscopy imaging of novel self-assembling pectin-liposome nanocomplexes. Carbohydr Polym 71 (2) 324—329 Takeuchi H, Yamamoto H, Niwa T, Hino T, Kawashima Y (1994) Mucoadhesion of polymer-coated liposomes to rat intestine in vitro. Chem Pharm Bull 42(9) 1954-1956 Takeuchi H, Yamamoto H, Niwa K, Hino T, Kawashima Y (1996) Enteral absorption of insulin in rats from mucoadhesive chitosan-coated liposomes. Pharm Res 13 896-901 Takeuchi H, Kojima H, Yamamoto H, Kawashima Y (2000) Polymer coating of liposomes with a modified polyvinyl alcohol and their systemic circulation and RES uptake in rats. J Control Rel 68(2) 195-205... 

Thirawong N, Thongborisute J, Takeuchi H, Sriamornsak P (2008b) Improved intestinal absorption of calcitonin by mucoadhesive dehvery of novel pectin-liposome nanocomplexes. J Control Rel 125(3) 236-245... 

Fig. 6 PEG-assisted DNA solubilization in DMSO and preparation of PEG/DNA nanogels (a) Plasmid DNA in water, (b) PEG/DNA nanocomplex in DMSO, and (c) stable DTME-crosslinked PEG/DNA nanogel in water. Reprinted from [128] with permission. Copyright 2006 American Chemical Society...

The preparation of disulfide-stabilized nanogel PEG/DNA complexes with a diameter of around 100 nm was reported by Mok et al. [128], A thiol-functionalized six-arm branched PEG was used for DNA solubilization in DMSO, which led to spontaneous PEG/DNA nanocomplex formation. Subsequent addition of the thiol-reactive dithio-bis-maleimidoethane (DTME) disulfide crosslinker led to stabilized nanogels (Fig. 6) that showed high gene transfection efficiency. 

Cross, K.J., Huq, N.L., Palamara, J.E., Perich, J.W., and Reynolds, E.C. (2005). Physicochemical characterization of casein phosphopeptide-amorphous calcium phosphate nanocomplexes. J. Biol. Chem. 280, 15362-15369. 

Actively transported PEI/DNA nanocomplexes exhibited an average velocity of 0.2 pm/sec [118], a value on the same order of magnitude as motor-protein driven motion. Transport was revealed to be microtubule dependent, because both active transport and perinuclear accumulation were abolished upon microtubule depolymerization. Experiments utilizing MPT to quantify the other intracellular barriers to gene delivery are under way. 

Har-el Y, Janardhana D, Hanes J. Enhanced transfection efficiency through longterm delivery of PEI/DNA nanocomplexes from porous PLGA microspheres. In preparation. 

The nanocomplexes formed between proteins and polysaccharides, and emulsion-based systems have been examined for their ability to protect and deliver a variety of food components. Cross-linking biopolymers has been used to improve microcapsule stability. Glutaraldehyde is an effective cross-linking agent but is not allowed in food. An alternative to glutaraldehyde that has been examined is tannin. 

An interesting example which capitalizes on the ability of the hydro-phobic cavity of (3-lactoglobulin to carry a hydrophobic molecules and complexation has been recently reported (Zimet and Livney, 2009). It was found that (S-lactoglobulin binds docosahexaenoic acid (DHA) and further that DHA-loaded (S-lactoglobulin can form nanocomplexes ( 100 nm) with low methoxy pectin at a pH colloidal stability and protected DHA against oxidation. 

Dot-siRNA nanocomplexes EVP-0015962 Fused oxadiazepines Geraniin Ginsenosides CK FI R41 Rh2... 

A more sophisticated alternative for inhibition of BACE1 is delivery of quantum Dot-siRNA nanocomplexes for BACE1 gene silencing [407],... 

Fig. 4 Synthesis of chitosan-PEG-FA (a), and electrospinning process for generating adenoviral (Ad)/chitosan-PEG-FA nanocomplexes (b)...

The present discussion [47] deals with the formation of natural self-assembly of CuS nanoclusters in dielectric substrates of gum Arabica biopolymer. Low concentration of ammonium complex of copper oxide solution was dissolved in gum Acacia Arabica solution at 60°C and stirred. H2S gas was passed in the same environment for 1 minute. Heating the resulting solution to about 100°C evaporates any possible trace of ammonia. The resulting nanocomplex was caste in the form of very thin film by spin coating technique. The developed specimen was used for experimental investigations, namely TEM, XRD, and electrical experiments such as impedance spectroscopy, and the Arrhenius plot and I-V characteristics were measured in the applied field direction perpendicular to the 2-D plane. 

Figure 12.31 AFM picture of ZnS Nanocomplex specimen of Gum Arabica [63bj.

Mao S, Germershaus O, Fischer D, et al. (2005). Uptake and Transport of PEG-Graft-Trimethyl-Chitosan Copolymer-Insulin Nanocomplexes by Epithelial Cells. Pharmac. Res., In Press. 

Fig. 4 Transfection process of TMC-Cys nanocomplexes (NC) including cell binding through electrostatic affinity and disulfide bonding, uptake via endocytosis, endosomal escape, intracellular plasmid DNA release, and nuclear transport of plasmid DNA [39]...

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