NADPH sensors

Self-supported photoresponsive MIP membranes synthesised using a photosensitive monomer, p-phenylazo-acrylanilide, and dansylamine as template, have been applied as recognition element in sensors [207, 208]. The binding capacity of the membrane for dansylamide could be reversibly modified by changing the illumination of membrane. Other examples are NADH and NADPH imprinted membranes... 

X.W. Fu et al., NADPH oxidase is an O2 sensor in airway chemoreceptors evidence from current modulation in wild-type and oxidase-deficient mice, Proc. Natl. Acad. Sci. USA 97 (2000) 4374-4379. 

Porwol T, Ehleben W, Brand V, Acker H, 2001. Tissue oxygen sensor function of NADPH oxidase isoforms, an unusual cytochrome aa, and reactive oxygen species. Respir Physiol 128 331-348,... 

Some of the above-mentioned characteristics of HIS biosensors can be demonstrated by considering the application of the liver microsomal NADPH oxidase activity in such sensors. The coupling of gelatin-immo-... 

The widespread use of dehydrogenase reactions in clinical chemistry prompted further investigation of the coupling of other enzymes with the microsomal NADPH oxidase in hybrid electrodes (Schubert et al., 1982b). Thus, sensors for ATP and isocitrate have been assembled (Table 15), which indicates the usefulness of this concept. 

The development of chemically modified electrodes, CMEs, has now reached a level of maturity which allows the inclusion of such electrochemical cells into various sensing devices. Special interest has been focused on the electron transfer or rather the hindrances for electron transfer during bioelectrochemical reactions. Studies of the oxidation of the reduced form of the nicotinamide coenzymes NADH and NADPH are attractive because a single electrochemical transducer reaction can be combined with any of the great number of dehydrogenases to give a sensor with the desired selectivity. 

The rat carotid body possesses an NADPH oxidase, which shows certain properties similar to the ones of NADPH oxidase in neutrophils (Acker et al. 1989). NADPH oxidase of the carotid body has been suggested to play a role as sensor for the oxygen concentration in the arterial blood (Acker et al. 1989). The rat carotid body shows a typical spectrum of cytochrome b and diphenylene iodo-nium inhibits H2O2 formation (Cross et al. 1990). 

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