NADPH production

Based on the difference in the rate of NADPH production with both glucose-6-phosphate and 6-phosphogluconolactone as substrates and the rate of NADPH production with only 6-phos-phogluconolactone as its substrate (107). 

Glucose 6-phosphate dehydrogenase, the first enzyme in the oxidative pentose phosphate pathway, is also regulated by this light-driven reduction mechanism, but in the opposite sense. During the day, when photosynthesis produces plenty of NADPH, this enzyme is not needed for NADPH production. Reduction of a critical disulfide bond by electrons from ferredoxin inactivates the enzyme. 

NADPH production (pentose phosphate pathway malic enzyme)... 

Rat liver mitochondria were isolated as described (4). The initial rate of ATP synthesis associated with the oxidation of succinate was followed by monitoring fluorometrically the ATP-linked NADPH production in the presence of hexokinase and glucose-6-phosphate dehydrogenase (10). Control experiments showed no interference from unexpected reduction of NADP+ or from electron backflow. Possible ATP formation via mitochondrial adenylate... 

To compute the maximal NADPH production per glucose consumed, we pose the following linear optimization problem maximize J43 (the NADPH output flux)... 

The computed optimal NADPH production is 12 molecules produced for every glucose molecule consumed. The flux distribution at this optimal solution is illustrated in Figure 9.5. 

Figure 9.5 Flux values in model of Varma and Palsson [204] corresponding to maximal NADPH production per glucose consumed. Figure is from [204],...

As red blood cells do not have mitochondria or a Krebs cycle, their energy is derived from glycolysis (ATP production) and from the HMP shunt (NADPH production). Defects either in glycolysis or in the HMP shunt may result in red ceU abnorm ities. Sometimes the defect centers in red blood cells. In other cases, it is more generalized. as glycolysis and the HMP shunt are generalized processes. Conditions that affect glycolysis or the HMP shunt include conditions 1-10). 

Regulation of NADPH Production Fatty Acid Synthase Respiratory Quotient Data from Human Studies... 

Larochelle, M.. Drouin, S.. Robert, F., and Turcotte, B. (2006) Oxidative stress-activated zinc cluster protein StbSp has dual activator/repressor fiinctions required for pentose phosphate pathway regulation and NADPH production. Molecular and Cellular Biology. 26. 6690-6701. 

Be familiar with how thiamine deficiency results in decreased energy generation and how it results in decreased ribose and NADPH production. 

Vander Jagt, D. L., Hunsaker, L. A., Kibirige, M., and Campos, N. M. (1989). NADPH production by the malarial parasite Plasmodium falciparum. Blood 74, 471 474. 

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