Myosin technique

As ATP binding to myosin, and ATP hydrolysis, are both faster than the overall observed ATPase rate, the slow step that follows the rapid phosphate burst and that must limit the overall observed ATPase rate must be the release of phosphate, or the release of ADP. The rate at which ADP is released was measured by a displacement technique (Trentham et al., 1972), in which the rate at which ADP bound to S-1 is displaced by ATP was measured. This experiment showed that the rate of ADP release (2 s ) is greater than the overall rate of hydrolysis (0.03 s" ). Thus the release of Pj, and not that of ADP, is rate limiting. 

One of the tasks of structural biologists studying muscle contraction is to determine the organization and shapes of the myosin head in muscle under different physiological conditions. The technique of low-angle X-ray diffraction has unique advantages in this process, particularly since it can be applied to living muscle, which can be stimulated to produce active force or can be studied under a variety of different steady-state conditions. The main problem with X-ray fiber diffraction, as detailed in Squire and... 

Because insect eggs do not contain myosin, the ELISA technique cannot detect infestation that contains only the egg stage. Johnson et al. (1973), however, claimed that fruit fly eggs can be detected by serological methods. The authors reported that the fruit fly eggs in fruit juices are detected by solid-phase radioimmunoassay as described by Johnson et al. (1971). 

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