Muscle scanning electron microscopy

Comparison of results of scanning electron microscopy and MRI was done before and after administration of a radiographic contrast agent in the tendon of the deep digital flexor muscle obtained from horse cadavers. 

To answer the question whether heterotopically formed bone, induced by hydroxyapatite, oc- and [3-TCP and biphasic HAp/TCP (BCP), would disappear over time due to the absence of mechanical stresses and whether this heterotopically formed bone would give rise to uncontrolled growth, a long-time investigation over 2.5 years was conducted (Yuan et al., 2001). Porous hydroxyapatite ceramic (HAp), porous biphasic calcium phosphate ceramic (TCP/HAp, BCP), porous a-TCP ceramic and porous P-TCP ceramic were implanted in the dorsal muscles of dog with an observation time of 2.5 years. Histological observation, backscattered scanning electron microscopy observation and histomorphometric analysis were performed on thin non-decalcified sections of retrieved samples. Normal compact bone with bone marrow was found in... 

Inoue T (1990) The three-dimensional ultrastructure of intracellular organization of smooth muscle cells by scanning electron microscopy. Im Motta PM (ed) Ultrastructure of Smooth Muscle. Kluwer Academic Publishers, Boston, pp 6377 Itakura S, Yamakawa H, Toyoshima YY, Ishijima A, Kojima T, Harada Y, Yanagida T, Wakabayashi T, Sutoh K (1993) Force-generating domain of myosin motor. Bio-chem Biophys Res Commun 196 15041510... 

Voyle, C. A. (1969). Some observations of cold-shortened muscle. J. Food Technol. 4, 275. Voyle, C. A. (1981). Scanning electron microscopy in meat science. Scanning Electron Microsc. 3, 405. 

Fig. 12.4. An oblique view of a fractured muscle fiber scanning electron microscopy at — 180°C (Sargent, 1988)...

In this study we have used a combination of scanning electron microscopy, transmission electron microscopy, immunoelectron microscopy and immunocytochemistry to determine the earliest pathological changes in skeletal muscle following the inoculation of notexin and to study the distribution of toxin binding sites. 

The length of the antiparallel overlap between molecules in a side-polar filament is not firmly established. A 43-nm antiparallel overlap was unique to segments formed from smooth muscle myosin rods by precipitation with divalent cations (Kendrick-Jones et al., 1971). Folded dimers with an approximately 40- to 50-nm antiparallel overlap are formed at salt concentrations <50 mM KCl (Trybus and Lowey, 1984). Both of these observations suggest that the molecule favors a 43-nm overlap. Measurements obtained by scanning transmission electron microscopy, however, show that filaments formed in vitro have one antiparallel dimer per 14.3 nm of filament length, a value that favors a model where adjacent molecules have a 14.3-nm antiparallel overlap (Cross and Engel, 1991) (Fig. 4). The... 

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