Multiple ligand binding sites

The affinity (Kj values) observed for [ H]MDA and [ HJMDMA binding were similar to the effective doses (i.e., ED50 or K] values) of MDA and MDMA reported for various pre- and postsynaptic monoamine markers, such as serotonin and dopamine release (Johnson et al. 1986), monoamine transport (Steele et al. 1987), and multiple brain, ligand binding sites (Battaglia et al. 1988). 

Fig. 6.3 Model ofthe domain structure ofthe natriuretic peptide receptor NPR, a receptor-type guanylyl cyclase. NPR is a dimeric transmembrane receptor which spans the membrane with two transmembrane elements. The extracytosolic domain comprises the ligand binding site and contains several disulfide bridges. The cytosolic part is composed of a kinase homology domain with multiple phosphorylation sites, an ATP binding site of unknown function and the catalytic guanylyl cyclase domain.

Fig. 5 Benefit of obtaining multiple crystal forms to aid with protein-ligand crystallographic work. Different crystal forms of mutant RadA arranged from the most open (left) to the most occluded (right) binding site (Marsh and Hyvonen, manuscript in preparation). Atoms involved in crystal contacts are coloured red and atoms that comprise the ligand binding site are shown in blue. The crystal symmetry mates closest to the binding sites are shown as cartoons. Space groups are indicated...

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