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Multiple-density-process

In this paper the fundamental aspects of process development for the production of core and virus-like particles with baculovirus infected insect cells are reviewed. The issues addressed include particle formation and monomer composition, chemical and physical conditions for optimal cell growth, baculovirus replication and product expression, multiplicity of infection strategy, and scale-up of the process. Study of the differences in the metabolic requirements of infected and non-infected cells is necessary for high cell density processes. In the bioreactor, the specific oxygen uptake rate (OURsp) plays a central role in process scale-up, leading to the specification of the bioreactor operational parameters. Shear stress can also be an important variable for bioreactor operation due to its influence on cell growth and product expression. [Pg.183]

Water Flux The permeability of a UF membrane is determined by pore size, pore density, and the thickness of the membrane active layer. Water flux is measured in the absence of solute, generally on a newly made or freshly cleaned sample. The test is simple, and involves passing water through the membrane generally in dead-end flow under carefully controlled conditions. In a water flux test, the membrane behaves as a porous medium with the flow described by Darcy s law. Adjustments for viscosity and pressure are made to correct tne results to standard conditions, typically the viscosity of water at 25°C and the pressure to 50 psi (343 kPa). The water flux will be many multiples ofthe process flux when the membrane is being used for a separation. Virgin membrane has a standard water flux of over 1 mm/sec. By the time the membrane is incorporated into a device and used in an application, that flux drops to perhaps 100 pm/s. Process fluxes are much lower. [Pg.1796]

Features corresponding to fixed kinetic energies, located at fixed positions from the end of the spectrum, are assigned to the accumulation of electrons relaxed in high density of state levels in the conduction band, above the vacuum level following the multiple relaxation processes suffered by incident electrons. [Pg.323]

In dealing with dilute systems and not too intense light sources one can eliminate, from the current density operator, the term with the vector potential A f). This eliminates from consideration multiple scattering processes and inelastic processes. The propagator ((Jx k ), jx(,k)))E is then approximated with its counterpart derived from the equations of motion of matter (electrons). [Pg.82]

The volume of hydrogen gas generated during anodization can be significant (Motohashi 2000), especially so when high current density is used and for simultaneous/multiple wafer processing. The... [Pg.567]

The explanation appears to lie in the accumulation of lattice vacancies at the nucleation sites of the helium bubbles. The average energy of a neutron in the fast reactor is above 100 keV, greatly in excess of the 25 eV or so which is required to displace an atom from a lattice site. Neutron collisions, followed by multiple cascade processes, therefore lead to a high density of vacancies and interstitials. The interstitials have a higher mobility than the vacancies and tend to be more rapidly absorbed at grain boundaries and dislocations, where they lose their identity. The surplus vacancies are then available for the formation of voids at the nucleation centers of the helium produced by the (n, a) reactions. [Pg.166]

In the second method, going beyond Bom, we examined the density of states within the coherent potential approximation (CPA) which takes into account multiple scattering processes. One might think that on this level impurity states are introduced in the gap. However, we find [16] that the existence of such localized impurity states strongly depends on the relative strength of site vs. bond impurity. Only states in the gap due to disorder can be found if the site amplitude f/s is stronger than the bond amplitude Ufc. Since CPA is an effective medium theory this result might be questionable in one dimension. [Pg.117]

By comparison, the multiple spinnerette per bank process requites additional effort prior to laydown in order to compensate for the gaps between the individual spinnerettes. Failure to present a filament array to the laydown screen, which is not uniformly distributed, can result in spot-to-spot variations in fiber density and a web that has the appearance of blotch. [Pg.167]


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See also in sourсe #XX -- [ Pg.189 ]




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Density multiplication

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