Endonuclease, restriction, recognition

Figure 9.3. Restriction map produced by Webcutter. The partial restriction map shows the nucleotide sequence of human lysozyme gene submitted to Webcutter using options for all restriction endonucleases with recognition sites equal to or greater than six nucleotides long and cutting the sequence 2 6 times (at least 2 times and at most 6 times). The restriction profile (map) is returned if Map of restriction sites is selected for display. The tables by enzyme name and by base pair number can be also returned if displays for Table of sites, sorted alphabetically by enzyme name Table of sites, sorted sequentially by base pair number are chosen.

The following is a section of the gene coding for bovine rhodopsin, along with a table listing several restriction endonucleases, their recognition sequences, and their hydrolysis sites ... 

Table 11.5 lists many of the commonly used restriction endonucleases and their recognition sites. Because these sites all have twofold symmetry, only the sequence on one strand needs to be designated. 

FIGURE 13.3 Restriction endonuclease Ec691 cleaves double-stranded DNA. The recognition site for is the hexameric sequence GAATTC ... 

Most useful restriction enzymes cut DNA at specific recognition sites, usually four to six nucleotides in length. There can be multiple restriction sites for a single endonuclease within a given piece of DNA, there can be only one (a unique restriction site), or there can be none. It all depends on the sequence of the specific piece of DNA in question. 

Table 13.2 Recognition sequences of a selection of restriction endonucleases. The double-stranded DNA is cut at the positions arrowed...

Table A4.1 presents the origins and sequence recognition sites for the restriction endonucleases in the plasmid vector.

This Is the DNA sequence recognized by the restriction endonuclease EcoRI. Rgure 1-6-1. EcoRI Recognition Sequence... 

Mutations in restriction sites (the recognition sites for restriction endonucleases such asMsfll)... 

Alul One of a family of restriction endonucleases that are site-specific endodeoxyribonucleases that cause cleavage of both strands of DNA within or near a specific site recognized by the enzyme its recognition sequence is AG/CT. 

Balendiran, K., Bonventre, J., Knott, R., Jack, W., Benner, J., SchUdkraut, 1. and Anderson, J. E. (1994). Expression, purification, and crystallization of restriction endonuclease Pvull with DNA containing its recognition site. Proteins 19,77-79. 

Design and synthesize two primers, an N-terminal primer and a C-terminal primer, for amplification of protein coding region of the target cDNAs. The N-Terminal primer should have the initiation codon at its 5 -terminus. In the case of the C-terminal primer, the restriction endonuclease recognition sequence should be introduced upstream of the stop codon, and an additional sequence (at least two bases) should be added to the 5 -end (rccNote 4). 

The recognition sequences of type II restriction endonucleases are usually symmetric they have a twofold axis of symmetry so that they read the same in the 5 -to-3 sense along opposite—but, of course, complementary—strands. Such sequences are... 

Thousands of restriction endonucleases have been discovered in different bacterial species, and more than 100 different DNA sequences are recognized by one or more of these enzymes. The recognition sequences are usually 4 to 6 bp long and palindromic (see Fig. 8-20). Table 9-2 lists sequences recognized by a few type II restriction endonucleases. In some cases, the interaction between a restriction endonuclease and its target sequence has been elucidated in exquisite molecular detail for example, Figure 9-2 shows the complex of the type II restriction endonuclease EcoRV and its target sequence. 

TABLE 9-2 Recognition Sequences for Some Type II Restriction Endonucleases... 

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