Protein multidimensional chromatography

Other reviews of multidimensional separations have been published. These include a book on polymer characterization by hyphenated and multidimensional techniques (Provder et al., 1995), a review on polymer analysis by 2DLC (van der Horst and Schoenmakers, 2003), and two reviews on two-dimensional techniques in peptide and protein separations (Issaq et al., 2005 Stroink et al., 2005). Reviews on multidimensional separations in biomedical and pharmaceutical analysis (Dixon et al. 2006) and multidimensional column selectivity (Jandera, 2006) were recently published. Suggested nomenclature and conventions for comprehensive multidimensional chromatography were published in 2003 (Schoenmakers et al., 2003), and a book chapter in the Advances in Chromatography series on MDLC was published in 2006 (Shalliker and Gray 2006). 

Lohaus, C., Nolte, A., Blueggel, M., Scheer, C., Klose, J., Gobom, J., Schueler, A., Wiebrin-ghaus, T., Meyer, H.E., Marcus, K. (2007). Multidimensional chromatography a powerful tool for the analysis of membrane proteins in mouse brain. J. Proteome Res. 6(1), 105-113. 

Liu, H. J., Berger, S. J., Chakrahorty, A. B., Plumh, R. S., Cohen, S. A. (2002). Multidimensional chromatography coupled to electrospray ionization time-of-fhght mass spectrometry as an alternative to two-dimensional gels for the identification and analysis of complex mixtures of intact proteins. J. Chromatogr. B 782(1-2), 267-289. 

Peng, J., Elias, J.E., Thoreen, C.C., Licklider, L.J., Gygi, S.P. (2003). Evaluation of multidimensional chromatography coupled with tandem mass spectrometry (LC/LC—MS/MS) for large-scale protein analysis the yeast proteome. J. Proteome Res. 2, 43-50. 

Apffel, A. (2004). Multidimensional chromatography of intact proteins. In Simpson, R.J., editor, Purifying Proteins for Proteomics A Laboratory Manual. Cold Spring Harbor Laboratory Press Cold Spring Harbor, New York pp.75-100. 

We have purposely narrowed the scope of all multidimensional chromatography to those techniques that incorporate separations in the liquid phase and to those in which the use of the comprehensive mode prevails but is not exclusive. This text neither incorporates elements of multidimensional thin-layer chromatography, multidimensional separations in gel media such as those commonly employed for the separation of complex mixtures of proteins, nor the techniques that utilize multidimensional gas chromatography. Some of the same principles apply, particularly in the theory section, but our emphasis is strictly on separations carried out in the liquid phase and by columns, rather than in the gas phase or in planar configurations. 

Hansen, K.C., Schmitt-Ulms, G., Chalkley, R.J., Hirsch, J., Baldwin, M.A., and Burlingame, A.L. (2003) Mass spectrometric analysis of protein mixtures at low levels using cleavable 13C-isotope-coded affinity tag and multidimensional chromatography. Mol. Cell. Proteomics 2, 299-314. 

J. Peng, et al., Evaluation of Multidimensional Chromatography Coupled with Tandem Mass Spectrometry (LC/LC-MS/MS) for Large-Scale Protein Analysis The Yeast Proteome. J. Proteome Res., 2, no. 1 (2003) 43-50. 

Figure 4.8. MuDPIT platform. The MuDPIT or multidimensional gel protein identification system is an multidimensional liquid chromatography (LC)-based system of separation prior to tandem mass spectrometry (MS/MS). It is not necessary to purify proteins prior MuDPIT, although a reduction in protein complexity by some prior purification is helpful in obtaining interpretable spectra. Protein lysates are digested into peptides that are loaded onto a strong cation exchange (SCX) support. Peptides are sequentially eluted onto a reverse-phase column for a second separation. Eluted peptides from the reverse-phase column are electrosprayed into a tandem mass spectrometer for amino acid sequencing and identification of proteins in the sample.

Gygi, S.P., Rist, B., Griffin, T.J., Eng, J. and Aebersold, R. (2002) Proteome analysis of low-abundance proteins using multidimensional chromatography and isotope-coded affinity tags. J. Proteome Res. 

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