Mouse ascorbic acid effect

Ota et al. (07) demonstrated that effects such as ovulation induced by human chorionic gonadotropin (HCG) in the mouse and the decrease in ovarian ascorbic acid levels caused by LH (P7) could be inhibited... 

Bates, C. J.. Bailey. A. J., Prynne, C. J.. and Levene. C. I. The effect of ascorbic acid on the synthesis of collagen precursor secreted by 3T6 mouse fibroblasts in culture. Biochim. Biophys. Acta. 278 372-390, 1972. 

Bromo-6-deoxy-L-ascorbic acid (480 mg per kg body weight) was capable of lowering the toxicity of 10 mg cisplatin per kg mouse (Sverko et al. 1999). In mice treated wilii higher doses of cisplatin (15 mg per kg body weight) 6-bromo-6-deoxy-L-ascorbic acid acerbated the toxic effects of cisplatin. 

MC3T3-E1, one of the available mouse osteoblastic cell lines, has been proved to be a model system suitable for analysis of the stimulatory effects of ascorbic acid on osteoblastic differentiation (Sudo et aL, 1983 Quarles et al., 1992), since MC3T3-E1 expresses osteoblast marker proteins and forms a mineralized extracellular matrix in response to ascorbic acid (Franceschi and Iyer, 1992 Torii et ai, 1994). Without ascorbic acid in the medium, the expression of alkaline phosphatase and osteocalcin mRNAs is totally repressed for a ten-day culture period (Fig. 2). On culturing with ascorbic acid, expression of alkaline phosphatase mRNA as well as enzyme activity begins to increase on day five, reaches a maximum level on day ten, and then decreases to the same level as that on day five. Similarly, osteocalcin is markedly induced only on culturing with ascorbic acid and reaches a maximum... 

With regard to the mechanism of its possible antiviral activity, we have observed an increase in interferon production by ascorbic acid following stimulation of mouse L cell cultures with poly (rI) poly (rC) (Siegel, 1975). We have also noted an enhancement of interferon production vivo following stimulation with Rauscher leukemia virus in BALB/c mice treated with 250 mg% L-ascorbic acid in the drinking water (Siegel, 1974). Thus, the antiviral activity of ascorbic acid may be due, in part, to enhanced interferon production however, the mechanism of this effect remains to be elucidated. 

Mirvish, S.S., A. Cardesa, L. Wallcave, and P. Shubik. 1975. Induction of mouse lung adenomas by amines or ureas plus nitrite and by N-nitroso compounds effect of ascorbate, gallis acid, thio cyanate, and caffeine.. Natl. Cancer Inst. 55 633-636. 

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