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Mounts, Canada balsam

Mount with xylene-based mounting medium, like Canada balsam or DPX however, aqueous medium can also be used (see Sect. 3.5). [Pg.66]

Carefully extracted samples of peat were slowly dehydrated in a series of alcohol solutions and then embedded in paraffin. After embedding, thin-sections (15 microns in thickness) were cut from these samples with a sliding microtome and mounted in Canada Balsam. Details of the procedure for the embedding and sectioning of peats have previously been described by Cohen.(2,3). [Pg.29]

Incubate dry slides for 1 h at 50°C, bring back to room temperature and mount in Canada balsam. [Pg.263]

Mounting media Glycerol, methyl salicylate, Permount or Canada balsam (see Notes 5 and 56). [Pg.78]

The final step in specimen preparation is to mount the prepared thin section, replica, fibers, or particulate strew, usually on a standard 25 mm x 76 mm (lin. x3in.) glass slide, with a mounting material (mountant) (Canada balsam or its proprietary substitutes, glycerin, oils, aqueous liquids), and then to cover the preparation with a coverslip, a thin glass disk, or plate. [Pg.3138]

Coverslip using Canada balsam mounting media. [Pg.157]

Prolonged exposure of embryonic tissues to the fixative diminishes P-galactosidase activity, and enzyme activity becomes undetectable after more than 60 min in paraformaldehyde. When coverslipping sections, use Canada balsam, as other mounting media can cause the blue stain to fade. [Pg.162]

Impregnation. Porous samples and delicate materials should be impregnated. This is performed with the same media which will be used to cement the sample to the slide in a later step. The impregnating medium, consisting of epoxy resin or Canada balsam, should have a suitable index of refraction (close to 1.535), allowing the phases to be distinguished from one another by optical methods. In many cases, it is sufficient to simply lay the sample in the mounting medium and then allow capillary forces to... [Pg.140]

Procedure. A small filter paper is impregnated with nitric acid-molybdate solution. The mounted section or the polished area of the rock is warmed (the section should not be heated to the point that the Canada balsam becomes mobile) and pressed at once on the moist paper and left for 1 or 2 minutes. The paper is laid (with the surface of contact upward) on a second paper soaked in benzidine solution, so that the solution rises through the upper paper. The latter is then held over ammonia and an exact blue print is obtained of the crystals of apatite in the section. If the contours of the section have been drawn on the paper, the exact location of the crystals is revealed. Even the smallest crystals of apatite, of whose apatitic nature there may be some doubt, may be identified in this way, thus amplifying or even replacing optical examination. [Pg.585]

In paraffin and mlerotomed The sections vere deparaffInated In absolnte xylol and mounted In Canada Balsam ... [Pg.44]

Venice turpentine has been used in picture varnishes and as a tackifying resin in adhesives partly because it inaeases the flexibility of the film, though this effect will diminish as the monoterpenes evaporate. Canada balsam was widely used for mounting specimens on microscope shdes and as a glass adhesive, though again the evaporation leads to shrinkage and distortion. [Pg.264]

Canada Balsam methyl salicylate (2 1), or other mounting medium of choice... [Pg.330]

Mount under a coverslip in 2 1 Canada Balsam methyl salicylate, or other appropriate mounting medium. Seal the edges with nail polish. [Pg.332]

Protocol 36.3 Rapid Mounting op Adult Structures in FIoyer s Medium, 612 Protocol 36.4 Preparation and Mounting op Aduet Structures in Canada Balsam, 613 VIEWING CUTICLE PREPARATIONS, 614... [Pg.600]

To prepare fine museum-quality, permanent slides, it is best to mount specimens in Canada Balsam. Canada Balsam is usually supplied as a solution in xylene, although it is sometimes supplied solid and must be dissolved in xylene ( 60-65% [w/v]) before use. With use, the concentration of xylene in a stock of Canada Balsam decreases, and it is necessary to add more xylene. It is difficult to give precise recipes for Canada Balsam, because every user seems to prefer a slightly different viscosity. We tend to use a rather dilute solution of Canada Balsam so that it spreads easily and does not dry too rapidly while mounting specimens. The disadvantage is that there is actually less Balsam in a drop of the solution, and when dried, it may contract from the sides of the coverslip, sometimes even disturbing the specimen. Unfortunately, there is no substitute for experience when using Canada Balsam. [Pg.602]


See other pages where Mounts, Canada balsam is mentioned: [Pg.329]    [Pg.28]    [Pg.29]    [Pg.212]    [Pg.149]    [Pg.20]    [Pg.21]    [Pg.21]    [Pg.22]    [Pg.428]    [Pg.475]    [Pg.92]    [Pg.206]    [Pg.3138]    [Pg.3138]    [Pg.159]    [Pg.161]    [Pg.50]    [Pg.290]    [Pg.611]    [Pg.465]    [Pg.137]   
See also in sourсe #XX -- [ Pg.21 , Pg.22 ]




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