Monoclonal antibody immunoassay

Allen RL, Manning W, McKenzie KD, et al. 1992a. Development of a monoclonal antibody immunoassay for the detection of gasoline and diesel fuel in the environment. Assoc Am Railroads Contaminated Soils-Diesel Fuel Contamination Research Triangle Park NC. 

Panteghini M, Pagani F. Diagnostic value of measuring pancreatic isoamylase with a double-monoclonal antibody immunoassay in serum of hospitalized hyperamylasemic patients. J Clin Lab Analysis 1990 4 449-52. 

Rasmussen-Loff AG, Nanchahal K, Cuckle HS, Wald NJ> Hulten M, Leedham P, et al. Amniotic fluid acetylcholinesterase m the prenatal diagnosis of open neural tube defects and abdominal wall defects a comparison of gel electrophoresis and a monoclonal antibody immunoassay. Prenat Diagn 1990 10 449-59. 

Davio et al. (43) report efforts to obtain monoclonal antibodies (mAbs) to STX. Because STX is a small molecule of approximately 300 daltons, well below the size necessary for immunogenicity, a carrier molecule must be conjugated to the hapten (STX). This technique must minimize alterations of the antigenic form. For the anti-STX antibodies tested to date, the ratios of immunoassay response factor to pharmacological potency for various STX derivatives differ substantially, the immunoassay being virtually unresponsive to some of the common natural derivatives (44). 

Figure 13 Structures of haptens used for immunizing and coating antigens in a monoclonal antibody-based immunoassay for diuron. A sensitive assay was developed using coating hapten I that had the handle in a position different from the immunogen hapten. When the oxygen in the urea moiety of hapten I was replaced with a sulfur (hapten 11), increasing the heterology, even greater sensitivity was achieved...

Elms M. J., Bunce I. H., Bundesen P. G., et al. Measurement of crosslinked fibrin degradation products. An immunoassay using monoclonal antibodies. Thromb Haemost 1983 50,591 -4. 

The most common BTA test is an immunoassay-based assay that uses monoclonal antibodies to detect the presence of bladder tumor-associated antigen in urine. In clinical studies, the BTA test was compared with cytoscopy-voided urine for the detection of recurrent bladder cancer. The sensitivity of BTA appeared su-... 

Volume 92. Immunochemical Techniques (Part E Monoclonal Antibodies and General Immunoassay Methods)... 

Fujiwara, K., Matsumoto, N., Yagisawa, S., Tanimori, H., Kitagawa, T., Hirota, M., Hiratani, K., Fukushima, K., Tomonaga, A., Hara, K., and Yamamoto, K. (1988) Sandwich enzyme immunoassay of tumor-associated antigen sialosylated Lewisx using b-D-galactosidase coupled to a monoclonal antibody of IgM isotype./. Immunol. Meth. 112, 77-83. 

D. H. Chunga, Comparative study of three immunoassays based on monoclonal antibodies for detection of the pesticide parathion-methyl in real samples. Anal. Chim. Acta 511, 323-331 (2004). 

A.Y. Kolosova, J.H. Park, S.A. Eremin, S.J. Kang and D.H. Chung, Fluorescence polarization immunoassay based on a monoclonal antibody for the detection of the organophosphorus pesticide parathion-methyl. J. Agric. Food Chem. 51, 1107-1114 (2003). 

M. Franek, A. Deng, and V. Kolar, Performance characteristics for flow injection immunoassay using monoclonal antibodies against s-triazine and 2,4-D herbicides. Anal. Chim. Acta 412,19-27 (2000). 

Antibody array Polyclonal or monoclonal antibodies are arrayed and used to detect and quantify specific proteins in a biological sample. An antibody array is effectively a parallel series of miniature immunoassays... 

Generation of antibodies that can recognize and bind to specific viruses is straightforward. A sample of live or attenuated virus, or a purified component of the viral caspid, can be injected into animals to stimulate polyclonal antibody production (or to facilitate monoclonal antibody production by hybridoma technology). Harvested antibodies are then employed to develop specific immunoassays that can be used to screen test samples routinely for the presence of that specific virus. Immunoassays capable of detecting a wide range of viruses are available commercially. The sensitivity, ease, speed and relative inexpensiveness of these assays render them particularly attractive. 

Geng, D., Shankar, G., Schantz, A., Rajadhyaksha, M., Davis, H., and Wagner, C. 2005. Validation of immunoassays used to assess immunogenicity to therapeutic monoclonal antibodies. Journal of Pharmaceutical and Biomedical Analysis 39, 364-375. 

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