Monoammonium Glycyrrhizinate

Assay Not less than 22.0% and not more than 32.0% of monoammonium glycyrrhizinate (C42H65N016), calculated on the dried basis. 

Standard Solution Dissolve about 10 mg of Monoammonium Glycyrrhizinate Standard for analytical use (Sigma, or equivalent), accurately weighed, in 20 mL of a 1 1 (v/v) solution of acetonitrile water. Filter the solution through a 0.45- xm filter (Millipore, or equivalent). Prepare fresh daily. 

Note Correct the weight of the Monoammonium Glycyrrhizinate Standard taken for the percent loss on drying shown on its label. 

System Suitability Inject duplicate 10- xL portions of the Standard Solution into the chromatograph. The retention time of monoammonium glycyrrhizinate is approximately 6 min. Adjust the operating conditions if necessary. The mean standard deviation for replicate injections is not more than 2.0%. 

Procedure Separately inject, in duplicate, 10- xL volumes of the Standard Solution and the Assay Solution into the chromatograph, and determine the mean peak area for each solution. Calculate the percent monoammonium glycyrrhizinate, equivalent to C HgjNOjg, in the portion of sample taken by the formula... 

Monoammonium Glycyrrhizinate occurs as a white powder with an intensely sweet taste. It is obtained by extraction from ammoniated glycyrrhizin. It is soluble in ammonia water and is insoluble in glacial acetic acid. 

SYNS AMMONIATED GLYCYRRHIZIN AMMONIUM GLYCYRRHIZINATE a-D-GLUCOPYRANO-SIDURONIC ACID, (3- 3,20-P)-20-CARBOXY-l 1-0X0-30-NOROLEAN-12-EN-3-YL 2-0- 3-D-GLUC0PYRANUR0-NOSYL-, AMMONIATE MONOAMMONIUM GLYCYRRHIZINATE... 

Monoammonium glycyrrhizinate. See Ammonium glycyrrhizinate Ammonium glycyrrhizate... 

Glycyrrhizin monoammonium salt High concentrated solution without additive 50... 

Glycyrrhizic acid, ammonium salt Glycyrrhizic acid, monoammonium salt Glycyrrhizin, ammoniated MAG Monoammonium glycyrrhizate... 

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