Molecular recognition, electrochemical interface

Interface, the air-water, chirality and molecular recognition in monolayers at, 28, 45 Intermediates, reactive, study of, by electrochemical methods, 19, 131 Intermediates, tetrahedral, derived from carboxylic acids, spectroscopic detection and investigation of their properties, 21, 37 Intramolecular reactions, effective molarities for, 17, 183 Intramolecular reactions, of chain molecules, 22, 1... 

Interface, the air-water, chirality and molecular recognition in monolayers at, 28, 45 Intermediates, reactive, study of, by electrochemical methods, 19, 131... 

The use of these labels for DNA detection typically requires covalent attachment because the probe molecules lack intrinsic DNA-binding capabilities. However, access to probes whose structural and electrochemical properties can be chosen for optimal efficiency amply justifies the added sample preparation time. In many of these cases, the electrochemical processes used for readout do not involve the DNA/electrode interface per se. Rather, the molecular-recognition properties of DNA are exploited to recruit the DNA-bound redox probes to the surface for analysis by more traditional electrochemical techniques, such as enzymatic catalysis or stripping voltammetry. 

The development of a portable and rugged sensing device requires that the selective recognition element be directly interfaced to the physical transducer. In the case of electrochemical transducers based on artificial BLMs, this entails stabilization of the assembly onto an electrode. The stabilization method must allow the membrane to retain characteristics of molecular mobility and fluidity which are essential for transduction and should provide sufficient ruggedness to permit use over an extended period of time (several months) without severe alteration of the response characteristics of the membrane. 

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