Molecular probes immunological techniques

Considerable progress has been made in the past few years in our understanding of the mechanism of progesterone action. Advancements have been due in large part to the availability of new techniques for structural analysis, in particular the use of affinity, immunologic and molecular probes. Interesting questions remain. For example, PR are unique among steroid receptors in that they are composed of two... 

Many different techniques, such as bacteriological culture, DNA staining using fluorochrome and immunological or biochemical methods, are available to detect mycoplasma contamination (see section 1.6). However, none seem to be fully efficient, so a combination of different methods is often necessary. Molecular tools such as hybridization using rDNA gene probes or polymerase chain reaction (PCR) have been developed over the past few years. Several studies using 16S rDNA-based PCR concluded that PCR seems to be a very convenient method for routine detection of cell culture contaminations (Spaepen, 1992 Teyssou, 1993 van Kuppeveld, 1994). 

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