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Molecular-level electron microscope

A hindrance to the elucidation of membrane structure is, of course, the material itself. Membranes are rather intractable lipoprotein systems. Their lipid, protein, and carbohydrate contents are variable both quantitatively and qualitatively since they cannot be crystallized, a detailed analysis by x-ray diffraction is impossible, and since they do not form solutions, the use of hydrodynamic or light-scattering techniques is quite limited. Electron microscopy has been the major physical method, but it is becoming increasingly clear that the electron microscope, at least at present, is incapable by itself of clarifying membrane structure on the molecular level (47). Despite an extensive literature, there is no general... [Pg.267]

The actual solubilization limit depends on the temperature, the nature of surfactant, the concentration of water, and on the nature of the acid. Irrespective of size or the specific properties of the solubilized molecules, very little is known about the thermodynamics or the kinetics of the solubilization process. The association of the solute with the interface can be checked using techniques capable of yielding detailed microscopic information at the molecular level (e.g. NMR, ESR, fluorescence, hydrated electrons). [Pg.86]

The biochemist s best friend is Escherichia coli, an ordinarily harmless inhabitant of our intestinal tract. This bacterium is easy to grow in the laboratory and has become the best xmderstood organism at the molecular level. It may be regarded as a typical true bacterium or eubacterium. The cell of E. coli (Figs. 1-1,1-2) is a rod 2 pm long and 0.8 pm in diameter with a volume of 1 pm and a density of 1.1 g/cm. The mass is 1 x 10 g, i.e., 1 picogram (pg) or -0.7 x 10 daltons (Da) (see Box 1-B). It is about 100 times bigger than the smallest mycoplasma but the internal structure, as revealed by the electron microscope, resembles that of a mycoplasma. [Pg.3]

The approach used to obtain the EVB free-energy functionals (the Ag of Equation (7)) has been originally developed in Ref. 25 in order to provide the microscopic equivalent of the Marcus theory for electron transfer (ET) reactions.38 This approach allows one to explore the validity of the Marcus formula and the underlying linear response approximation (LRA) on a microscopic molecular level.39 While this point is now widely accepted by the ET community,40 the validity of the EVB as perhaps the most general tool in microscopic LFER studies is less appreciated. This issue will be addressed below. [Pg.269]

See phase (1) solid liquid gas. (2) Levels Matter is basically composed of particles in the following levels of size and complexity (a) subatomic (protons, neutrons, electrons) (b) atomic and molecular (below 10 A) (c) colloidal (from 10 A to 1 micron) (d) microscopic (e) macroscopic (f) space or celestial. Level (a) is invisible by any means levels (b) and (c) can be resolved in field-ion or electron microscopes level (d) lies in the range of the optical microscope (e) is visible to the naked eye (f) re-... [Pg.792]

There has been increasing interest in recent years in using incoherent electronic excitation transport as a probe of molecular interactions in solid state polymer systems. The macroscopic properties of such systems arise from the microscopic interaction of the individual polymer chains. The bulk properties of polymer blends are critically dependent on the mixing of blend components on a molecular level. Through the careful adjustment of the composition of blends technological advances in the engineering of polymer materials have been made. In order to understand these systems more fully, it is desirable to investigate the interactions... [Pg.323]


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Microscopic level

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