Molecular inducible nitric oxide synthase iNOS

Casanova There are patients with severe infectious diseases caused by many other microorganisms, and they haven t been investigated at the molecular genetic level. Perhaps these investigations will lead to the discovery of other pathways. Regarding inducible nitric oxide synthase (iNOS), I think it is expressed in humans too and we hope to identify human patients with iNOS deficiency some day. 

Most human melanoma tumors cells are known to express the enzyme, inducible nitric oxide synthase (iNOS), which is responsible for cytokine-induced nitric oxide (NO) production in macrophages during immune responses (Grimm et al. 2008). Molecular analysis supports the hypothesis that iNOS-produced NO in melanoma can be responsible for driving proliferation as well as regulating resistance to apoptosis this hypothesis is supported by results of experiments in which... 

The molecular mechanism linking the inflammatory response to redox equilibria and modification of nitric oxide production will be explored in an animal model system of septic shock, a generalized inflammation induced by bacterial lipopolisaccharide (LPS). It is known that endotoxemia induces a complex interplay between the activation of nuclear transcription factors such as nuclear factor kappa B (NFkB) and a cascade-activation of various enzymatic activities, mostly mediators of the inflammatory response with particular attention to the variation of the inducible form of nitric oxide synthase (iNOS). 

The nitric oxide (NO) synthases (NOSs) (L-arginine, NADPHroxygen oxidoreductases EC 1.14.13.39) constitute a family with at least three distinct isoforms. In order of their molecular characterization, these include the neuronal (nNOS, NOSl) (Bredt et al., 1991 Nakane et al., 1993), inducible (iNOS, NOSl) (Xie et al., 1992 Lyons et al., 1992 Lowenstein et al., 1992 Wood et al., 1993 Geller et al., 1993a), and endothelial constitutive (ecNOS, NOSS) (Lamas et al., 1992 Marsden et al., 1992 Sessa et al., 1992 Janssens et al., 1992) NOSs. Their apparent molecular masses are 160, 130, and 135 kDa, respectively. Originally purified from neurons, cytokine-induced macrophages, and vascular endothelium (hence the designations nNOS, iNOS, and ecNOS), the three NOS isoforms are now appreciated to distribute across a wide spectrum of cell types... 

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