Molecular biological study

Molecular biology studies have identified a loop containing 20-25 amino acid residues between S5 and S6 (or Ml and M2, Fig. 2) forming the pore. The G(Y/F) G motif located in the pore represents the K+-selectivity signature, which is common to all K+ channels. The external entry to the channel pore and its adjacent residues constitute binding sites for toxins and blockers. The internal vestibule of the pore and the adjacent residues in S5 and S6 contribute to binding sites for compounds such as 4-aminopyiidine and quinidine. The S4-S5 linker lies close to the permeation pathway and is required for... 

This working model would be the framework for the interpretation of all subsequent biochemical, spectroscopic, and molecular biological studies on this unknown protein. The discovery of a putative [6Fe-6S] protein may have been a surprise to some, but its existence in... 

Recently, molecular biology studies have been carried out on hepatic uptake transporters. With regard to the Na+-dependent hepatic uptake of bile acids, Na+-taurocholate cotransporting polypeptide (Ntcp/NTCP) has been cloned from both rodents and humans [14-17]. Ntcp/NTCP accepts bile salts, such as taurocholate and glycocholate, as well as some anionic compounds such as dehydroepian-drosterone sulfate and bromosulfophthalein [16, 18]. However, the presence of unidentified Na+-dependent transporters for anionic drugs (e.g., bumetanide) has also been suggested [19, 20]. 

Photonics technologies are used widely today for applications in disease diagnosis at the molecular level, as well as in molecular biology studies of disease. Often, the sensing technique involves the detection of photons emitted by... 

Szalai, C., Molnar, E., SaUay, P., and Czinner, A. (1998) Molecular biological studies on patients with nephrogenic diabetes insipidus. Orv. Hetil. 139, 883-887. 

Fig. 4.5.2 Actual strategies for CDG diagnosis. Initial investigations on CDG patients are routinely carried out by isoelectric focusing (IEF) of serum transferrin. With a CDG type I pattern, subsequent analysis should imply determination of phosphomannomutase (PMM) and phos-phomannose isomerase (PMI) activities. Further studies, like analysis of the lipid-linked- and protein-bound-oligosaccharides, determination of enzyme or sugar transporter activities and molecular biology studies often have to be performed in more specialised laboratories. HPLC High-performance liquid chromatography, TLC thin-layer chromatography...

A review focusing on recent genetic and molecular biological studies of the matrix proteoglycans. The structure-function relationships of some paradigmatic proteoglycans are discussed in depth, and novel aspects of their biology are examined. 

Molecular biological studies indicate that isoforms or splice variants exist in the human brain for genes encoding receptor subtypes of 5-HT2A (56), 5-HT2C (3,57), 5-HT3 (58-60), 5-HT4 (61,62), 5-HT6 (63), and 5-HT7 (64) receptors. 

I have been purposely nebulous so far on where the DNA is, and what sites of the DNA are involved. In order to be more concrete we require a short description of the molecular biology studies. 

The biosynthetic origin of marine natural products is a complex topic that has gained more clarity through application of molecular biological study microbial symbionts may represent the true source in many sponges, ascidians, and bryozoans, even in cases in which localization studies have suggested an invertebrate source for... 

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