Ml3 phage

Scarselli E, Esposito G, Traboni C, Receptor phage. Display of functional domains of the human high affinity IgE receptor on the Ml3 phage surface, FEBS Lett., 329 223-226, 1993. 

Vassart G, Georges M, Monsieur R, Brocas H, Lequarre AS, Christophe D (1987) A sequence in Ml3 phage detects hypervariable minisatellites in human and animal DNA. Science 235 683-684... 

Georgieva Y, Konthur Z (2011) Design and screening of Ml3 phage display cDNA libraries. Molecules 16(2) 1667-1681. doi 10.3390/moleculesl6021667... 

II ssDNA (-h) Duplex replicative form [Pg.1650]

Another viral chromosome that can be used as a vector is that of the filamentous phage Ml3. The M13 chromosome is a single-stranded DNA molecule which when inserted into the bacterial host replicates outside the bacterial chromosome in the cytoplasm. The virus is then reassembled and released from the bacterial cell without cell lysis. 

To achieve surface display, five of the Ml3 coat proteins have been used in fusion to foreign protein fragments. In the most widespread system the antibody is coupled to the N-terminus or second domain of the minor coat protein pill (11,12,14). The naive function of the three to five copies of the pill, in particular their N-terminal domain, is to provide binding of the phage to the f-pili of E. coli to initiate infection (39). The major coat protein (pVIII) has been used as an alternative fusion partner, with only very few successes reported in the past decade (40). This fusion technique is more useful for the display of short peptides (41,42). Fusions to pVI have been tried, but not yet with antibodies (43). pVII and pIX were... 

At the time of writing this chapter, three commercial random peptide display libraries are available. Two phage Ph.D. libraries (New England Biolabs) are constituted of random 7-mers and random 12-mers fused to pill protein of the filamentous phage Ml3. The FliTrx (Invitrogen) is an E. coli display library with constrained random dodeca-peptides inserted into flagellin-thioredoxin fusion protein [10]. 

Although a number of different phage (mid) antibody libraries have been published (see Table 18.1), the number which are naive is relatively small. To date, all phage antibody libraries have been made on the filamentous phage, Ff (which comprises fd, fl, and Ml3). In the creation of such libraries a number of different choices can be made ... 

Lambda phage is closely related to other bacteriophages, and these are called T3, T7, T5, Mu, T2, and T4. All of these phages corcsist of a double-stranded DNA genome, an icosahedral head, a long cylindrical tail, and a variable number of tail filaments. The related filamentous phages are Ml3, fl, and fd. 

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