Mitosis phase analysis

Morphometric analysis demonstrated that the area of hepatocytes in reference group animals varied within the range of 450 20 pm, and in control groups 460 25 pm. In animals that received ES or IPG, the area of hepatocytes amounted to 650 40 pm in both cases, i.e. authentically increased (p < 0.01) both in regard to reference and control. Total cell quantity in different mitosis phases amounted to 20 3% of total cell quantity in reference rats, 23 4% in control animals, and among rats that received ES or IPG the total amount of fissionable cells increased by 36% on... 

Fig. 9. The cell cycle. M mitosis G1+S+G2 interphase, i.e., the period between ceU division. During G1 (G for gap) the cellular genome is in the diploid state. This is followed by the S-phase (S for synthesis) during which the DNA is replicated, and finally G2. Insert Cell-cycle analysis of HM02 cells cultured with and without vinblastine. CeU-cycle distribution was determined by staining DNA with propidium iodide, and the number of cells in different phases of the cell cycle was measured with a FACStar flow cytometer. Vinblastine, which disrupts the formation of microtubules, causes cell-cycle arrest in the G2/M-phase...

Temin (1970) and Todaro et al. (1965) showed similar effects for chicken fibroblasts and 3T3 mouse fibroblasts. The low level of serum is important for survival as well as for the subsequent stimulation of DNA synthesis (Cherrington, 1984). A kinetic analysis using time lapse cinematography (Zetterberg and Larsson, 1985) showed that Swiss 3T3 cells were only susceptible to cell cycle arrest in a short period (3-4 h) following mitosis. Even a 1-h exposure to serum-free medium during this time forced the cells into GO from which they required 8 h to return to Gl. The length of the postmitotic sensitive phase was very constant at between 3 and 4 h but considerable intercellular variability existed in the duration of the pre S-phase Gl period consistent with a transition probability event ( 10.4). 

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