MISPE molecularly imprinted polymer

The application of MIPs as the stationary phase in solid-phase extraction (SPE), often referred to as molecularly imprinted polymer solid-phase extraction (MIS P E), is a rapidly growing area [197-199]. With MISPE, highly specific enrichment of substances present at trace levels is possible. The technique has been applied to the analysis of drugs, for example, caffeine [200], scopolamine [201], naproxen [202], tetracycline [203], cholesterol [204] and local anesthetics [205], as well as environmental pollutants, exemplified by organophosphate flame retardants [206-208], triazines in soil and vegetable samples [71] and naphthalene sulfonates in river water [209]. 

A molecularly imprinted column for liquid chromatography can be used not only to separate analytes, but also to selectively extract analytes from complex samples. This technique is called on-line Molecularly Imprinted Solid Phase Extraction (on-line MISPE), and it combines the high extraction efficiency of reverse phase SPE for aqueous samples with the high selectivity of the molecular-imprinted polymers. Examples of successful selective extraction and clean-up are reported in Figs. 9 and 10. 

Molecular imprinted polymer microspheres against enrofloxacin have been developed and off-line molecular imprinted solid-phase extraction (MISPE) has been applied for the extraction of enrofloxacin from bovine milk.t""i... 

Molecularly imprinted SPE (MISPE) is a type of SPE that is more time-consuming than simple SPE since the molecularly imprinted polymer (MIP) stationary phase has to be specifically synthesized for the target dye recognition. Recently a titania-based MIP was synthesized through a sol-gel process using Sunset Yellow as template, without use of a functional monomer [27]. This MIP was used as an SPE material for the isolation and enrichment of sulfonic acid dyes in beverages. The better cleanup ability demonstrated the capability of MIP for the isolation and enrichment of sulfonic acid dyes in complicated food samples. 

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