Mid-infrared Synchrotron Radiation FT-IR Studies of Cultured Cells

4 Mid-infrared Synchrotron Radiation FT-IR Studies of Cultured Cells 

Much of the in-vivo work in H N as well as other sites has been led by Stone (a co-author of Chapter 4 in this book). His preliminary work, on laryngeal tissue, published in 2000, used linear disciminant analysis to separate spectra with the most visible differences being seen at 850, 950, 1200 and 1350 cm. He also concluded that the relative intensity of the nucleic acid peak increases with progression to malignancy. Not only do several publications indicate efficacy in the differentiation of non-malignant and malignant cell 

Vibrational spectroscopy carries much potential for cancer diagnosis and the development of predictive and prognostic models. Much of this consideration of H N cancers can be generalized to other sites. The key message is that a basic understanding of the disease and its therapy facilitates scientific advances. Recent developments in technology make laboratory-based and even hand-held probes a feasible development within the next 5 10 years. If this promise is confirmed, vibrational spectroscopy will make a substantial contribution to clinical medicine. For this reason, careful collaboration between clinicians and spectroscopists forms the ideal base for robust research leading to results that are likely to form the basis for a dependable and reproducible dataset and to result in a direct clinical impact. 

The spectroscopic studies that are reported in this chapter were undertaken primarily at the Synchrotron Radiation Source (SRS), Daresbury Laboratory, 

Our recent work, in Leeds, has been funded by Cancer Research UK, who fund the research undertaken by Andrew Harris through a Clinical Research Training Fellowship. 

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