Microwave-enhanced Solid-phase Peptide Synthesis

Microwave-enhanced Solid-phase Peptide Synthesis 

With the involvement of peptides and proteins in virtually every biological process, understanding and controlling their expression and function is of major importance. With more than 20000 papers published annually, peptide research has gro vn dramatically in recent decades [1]. Peptides are involved in many biochemical processes including cell-cell communication, metabolism, immune response, and reproduction. Peptides also act as hormones and neurotransmitters in receptor-mediated signal transduction. As the enormous role of peptides in many physiological and biochemical processes has become more understood, so has interest in their value as potential drug candidates. 

Obtaining peptides from natural sources can often be very difficult. In tissue samples the desired peptides are often at very low concentrations requiring highly sensitive assay methods. The availability and storage of natural tissue samples can also limit availability. Although recombinant genetics has been the major production tool for synthesis of proteins, it is a time-consuming and laborious task laden with difficulties. 

Microwaves in Organic Synthesis, Second edition. Edited by A. Loupy Copyright 2006 WILEY-VCH Verlag GmbH Co. KGaA, Weinheim ISBN 3-527-31452-0 

Although chemical synthesis of peptides and proteins has developed substantially in recent decades, it does require use of toxic reagents, large amounts of expensive reagents, and often suffers from incomplete reactions that significantly reduce final product purity. Chemical synthesis can be performed in solution, on a solid phase, or by a combination of both. Solution-phase synthesis has been the method of choice for large-scale preparation, but developments in solid phase tools have enabled its use also. 

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Microwave-enhanced Solid-phase Peptide Synthesis... 

I 20 Microwave-enhanced Solid-phase Peptide Synthesis Tab. 20.1. Phosphonium and aminium activators. 

Coffins JM (2006) Microwave-enhanced solid-phase peptide synthesis. In Loupy A (ed) Microwaves in organic synthesis. Wiley-VCH, Weinheim... 

Yu, H.M., Chen, S.T. and Wang, K.T., Enhanced coupling efficiency in solid-phase peptide synthesis by microwave irradiation, /. Org. Chem., 1992, 57, 4781. 

Palasek, S.A., Cox, Z.J., and Collins, J.M. (2007) Limiting racemization and aspar-timide formation in microwave-enhanced Fmoc solid phase peptide synthesis. J. Pept. Sci. 13,143-148. 

Rodriguez H, Suarez M, Albericio F (2010) A convenient microwave-enhanced solid-phase synthesis of short chain N-methyl-rich peptides. J Pept Sci 16 136-140... 

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