Microtubule dynamics

Mitchison, T.J. (1988). Microtubule dynamics and kinetochore function in mitosis. Ann. Rev. Cell Biol. 4, 527-549. 

Gotoh, Y., Nishida, E Matsuda, S., Shiina, N., Kosako, H Shiokawa, K., Akiyama, T., Ohta, K., and Sakai, H. (1991 a). In vitro effects on microtubule dynamics of purified Xenopus M phase-activated MAP kinase. Nature 349 251-254. 

Belmont LD, Hyman AA, Sawin KE, Mitchison TJ (1990) Real-time visualization of cell cycle-dependent changes in microtubule dynamics in cytoplasmic extracts. Cell 62 579-589... 

Microfilament and microtubule dynamics underlie growth cone motility and function 132... 

Ixabepilone (38 Ixempra, BMS-247550 Bristol-Myers Squibb, 2007), a semi-synthetic derivative of epothilone B (39) produced by Sorangium cellulosum, was developed by Bristol-Myers Squibb (BMS) as an anticancer drug (administered through injection) that binds directly to (3-tubulin subunits on microtubules, leading to suppression of microtubule dynamics, blocking of cells in the mitotic phase and ultimately leading to... 

Although not a taxane, ixabepilone is a novel microtubule inhibitor that was recently approved for metastatic breast cancer in combination with the oral fluoropyrimidine capecitabine or as monotherapy. It is a semisynthetic analog of epothilone B, and is active in the M phase of the cell cycle. This agent binds directly to 6-tubulin subunits on microtubules, leading to inhibition of normal microtubule dynamics. Of note, this agent continues to have activity in drug-resistant tumors that overexpress P-glycoprotein or tubulin mutations. The main adverse effects include myelosuppression, hypersensitivity reactions, and neurotoxicity in the form of peripheral sensory neuropathy. 

Panda D, Rathinasamy K, Santra MK, Wilson L (2005) Kinetic Suppression of Microtubule Dynamic Instability by Griseofiilvin Implications for its Possible Use in the Treatment of Cancer. Proc Natl Acad Sci USA 102 9878... 

Orbit/MAST proteins, also known as CLIP-associated proteins (CLASPs), are involved in the regulation of microtubule dynamics and bind to microtubule plus ends via CLIP115 or CLIP170. Active CLASP suppresses microtubule assembly and axon outgrowth (Lee et al., 2004), whereas activated adenomatous polyposis coli protein (APC see below) promotes microtubule assembly and axon outgrowth. 

Cassimeris, L. (1999). Accessory protein regulation of microtubule dynamics throughout the cell cycle. Curr. Opin. CellBiol. 11, 134—141. 

Errico, A., Ballabio, A., and Rugarli, E. I. (2002). Spastin, the protein mutated in autosomal dominant hereditary spastic paraplegia, is involved in microtubule dynamics. Hum. Mol. Genet. 11, 153-163. 

Galjart, N., and Perez, F. (2003). A plus-end raft to control microtubule dynamics and function. Curr. Opin. Cell Biol. 15,48-53. 

Hamill, D. R., Howell, B., Cassimeris, L., and Suprenant, K. A. (1998). Purification of a WD repeat protein, EMAP, that promotes microtubule dynamics through an inhibition of rescue./ Biol. Chem. 273, 9285-9291. 

Hyman, A. A., Salser, S., Drechsel, D. N., Unwin, N., and Mitchison, T. J. (1992). Role of GTP hydrolysis in microtubule dynamics Information from a slowly hydrolyzable analogue, GMPCPP. Mol. Biol. Cell 3, 1155-1167. 

Kline-Smith, S. L., and Walczak, C. E. (2004). Mitotic spindle assembly and chromosome segregation Refocusing on microtubule dynamics. Mol. Cell. 15, 317-327. 

Rodionov, V., Nadezhdina, E., and Borisy, G. (1999). Centrosomal control of microtubule dynamics. Proc. Natl. Acad. Sci. USA 96, 115-120. 

Vaillant, A. R., Muller, R., Langkopf, A., and Brown, D. L. (1998). Characterization of the microtubule-binding domain of microtubule-associated protein 1A and its effects on microtubule dynamics./. Biol. Chem. 273, 13973-13981. 

Walczak, C. E., Mitchison, T. J., and Desai, A. (1996). XKCM1 A Xenopuskinesin-related protein that regulates microtubule dynamics during mitotic spindle assembly. Cell 84, 37-47. 

Paclitaxel is a well-established antiproliferative agent with a microtubule-targeting pharmacologic activity (37,42,43). As an anticancer agent, paclitaxel causes polymerization and stabilization of microtubules (43-45). The stabilization of microtubule dynamics by paclitaxel can interrupt many cellular processes, including cell division, migration, activation, maintenance of cytoskeletal framework, and intracellular as well as transmembrane protein transport (42,46,47). 

Keywords Cytoskeleton, Microtubule dynamics, Mitosis, Structure Vinca domain... 

Kodama A, Karakesisoglou I, Wong E, Vaezi A, Fuchs E. 2003. ACF7 An essential integrator of microtubule dynamics. Cell 115 343-354. 

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