Microscopy stereo microscope

Optical microscopy to observe volume changes was done with a Zeiss stereo microscope, using a 3 mm high round sample cell. 

The steps involved in the problem solving protocol are outlined in Table 7.1. They are rather simple and do not take much time to consider and such a protocol can save time in the long run. The protocol involves steps typical of scientific inquiry collect all the currently known facts, determine the nature of the problem, state the objective of the study, obtain the correct specimen, be sure to have experimental controls, look at the sample with the naked eye and then with a stereo microscope. These provide an aid to selection of the specific microscopy techniques and preparation methods needed to begin to address the objectives. The result should be that clearly defined analyses are conducted. 

The principles behind preparing microscopic samples for infrared analysis are similar to those for preparing macroscopic samples, as discussed in Chapter 4. Since an infrared microscope can be used in transmittance and reflectance, both types of sample preparation come into play. Unfortunately, the preparation of microscopic samples for infrared analysis may be the hardest part of using an infrared microscope. Frequently, samples are too small to be seen by the naked eye, so the preparation of samples for infrared microscopy requires the use of a low-power stereo microscope, as shown in Figure 6.5. 

Light microscopy allows, in comparison to other microscopic methods, quick, contact-free and non-destmctive access to the stmctures of materials, their surfaces and to dimensions and details of objects in the lateral size range down to about 0.2 pm. A variety of microscopes with different imaging and illumination systems has been constmcted and is conunercially available in order to satisfy special requirements. These include stereo, darkfield, polarization, phase contrast and fluorescence microscopes. 

Scanning electron microscopy (SEM), along with stereo or standard light microscopes, can be used to determine the morphology of materials on the surface of a membrane. 

The single most important preparation instrument in the microscopy laboratory is the stereo binocular microscope. These instruments are inexpensive and readily available. Materials may be observed in either transmitted or reflected light, and the result often provides insights into the problem. Even rather large parts may be examined as part of the important first step in choosing the area of a sample to be analyzed. 

T 2. Can optical microscopy provide an Yes First try a stereo binocular microscope to select a sample for... 

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