Micropipette puller

User s Manual for Micropipette Puller Model P-2000. Sutter Instruments, Novato, CA, 1998. M. V. Mirkin and A. J. Bard. Anal. Chem. 64 2293 (1992). 

We use 100 pi glass capillary tubes (VWR international 53432-921) pulled by a horizontal micropipette puller (Sutter Instrument Co. Model P-97) for suctioning of adipose tissue. Suction strength is controlled by tip size which is typically 10-20 pm. 

Instruments/equipment P-97 Micropipette Puller (Sutter Instruments), Borosilicate glass pipettes, Axon Multiclamp 700B microelectrode amplifier (Molecular Devices), Axon Digidata 1440A data acquisition system (Molecular Devices), pCLAMP 10 electrophysiology data acquisition and analysis software (Molecular Devices), Temperature Controller (TC-344B, Warner Instruments). 

Ultraminiaturized fiber optic sensors under 100 fim have only recently been fabricated. Tan et al [23, 24] developed a submicrometer optical fiber tip by pulling out silica fibers on a micropipette puller using a 25 W CO2 infrared laser as a heat source. Tips as small as 0.1 /xm could be reliably fabricated. After pulling, the tips were sputtered with aluminum in a vacuum chamber. This fabrication technique leaves a very small aperture at the tip, which can then be used as a near-field optical device (discussed in the next section). 

User s Manual for Micropipette Puller Model P-2000. Sutter Instruments, Novato, CA, 1998. 

Die constmction of nanosensors is cracially dependent on the constmction of nanometer-sized tips on optical fibers. Neai-field optical microscopy has spawned nanoscale optical fibers produced by either pulling with micropipette pullers or by chemical etching. 

Another technique prepares small diskshaped tips hy sealing metal wires in glass using a laser-based micropipette puller [25]. The metal was exposed either by etching the glass insulator or... 

Glass needles are made from Microcaps micropipets (Drununond Scientific Co., USA) using a vertical micropipette puller (David Kopf Instruments Model 720). 

Injection needles are prepared from S -in. hard glass capillaries (Drummond 3-000-203-G/X) using a Narashige PN-3 horizontal micropipette puller (Narishige International). 

Capillary pipette puller such as the Sutter Model 97 Flaming/ Brown micropipette puller. 

Fig. 2. Flaming/Brown micropipette puller (Flaming/Brown). A. full frame view with hd open to show top interior of puller. B. Capillary carriers with inserted capillary (arrow). Arrowhead points to box filament for heating glass. C. Two newly fabricated capillary micropipettes (arrows) after heating the glass to the point of rupture under tension. The safety cover over the box filament has been removed to give better visual access in the photographs.

Programmable Micropipette Puller, PMP-102 (MicroData Instrument Inc., Plainfield, NJ, USA). 

Pull micropipettes using a modified version of sequence 19 (Table 24.2) of the Programmable Micropipette Puller (PMP-102), set pressure 1 p) = 0.4 and pressure 2 p) = 1.5, and set the steps as follows see Note 14). The internal diameter of the micropipette should be 5-10 xm at the opening and it may be necessary to score the tip of the micropipette to achieve this diameter. 

As an example, the RSNOM instrument constructed by the authors is shown schematically in Fig. 31 [35,45,46]. In this implementation of the SNOM technique, the sub-A/2 aperture is used to illuminate the sample. This aperture is provided by a tapered fiber-optic probe that has been coated with aluminum such that a 100-200-nm aperture is defined at the apex of the taper. The tapered fiber-optic probes are melt-drawn using an adapted micropipette puller (Sutter Instruments, U.S.A.) and coated with approximately 100 nm of aluminum. During evaporation, the probes are held at an angle to the source and rotated to define the aperture. The aperture is held in the near field using the shear-force feedback technique. The amplitude of the probe s dithering motion is monitored using a simple optical detection scheme. When the probe moves to within approximately 30 nm of the... 

NanoESI capillaries were prepared from borosilicate glass capillaries (OD = 1.00 mm, ID = 0.78 mm, length = 10 cm) (Warner), using a Flaming/ Brown P-97 micropipette puller (Sutter Instruments). The pulled tip was then coated in gold for electrical conductivity using a vacuum evaporator built in-house... 

Nano-ESI capillaries were pulled using a Flaming/Brown P-97 micropipette puller (Sutter Instruments). In order to achieve a tip shape shown in Fig. 2.3, the parameters were carefully optimised by to obtain a satisfactory tip shape [7]. Although parameters can change when using different filaments, a typical set of values are detailed in Table 2.7. 

Table 2.7 Instrument parameters for Flaming/ Brown P-97 micropipette puller...

Several standard strategies for removing the chorion are available. We prefer chemical dechorionation by immersion in 50% commercial bleach (final concentration 2.5% hypochlorite). Although in principle, chorions can be cleared by immersion in halocarbon oil, an intact chorion is tough on the tips of standard glass micropipettes and the use of fused-quartz capillary stock and a laser micropipette puller may be necessary if the chorion is left intact. 

Pull micropipettes from capillary stock with a glass filament (e.g., 2-mm outer diameter and 0.68-mm inner diameter A-M Systems) using a standard micropipette puller. 

Micropipette puller e.g., P-97, Flaming/Brown type (Sutter Instrument, Novato, CA). 

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