Microbore tubing, applications

Ideally, a sample is introduced into a chromatograph as a perfect plug. In practice, this is not the case, and diffusion occurs because of the injector. For narrow-bore and microbore applications, injectors capable of introducing the required sample volumes are commercially available and optimized to reduce dispersion. This is not the case for capillary LC, and homemade injection systems include the sample tube technique, in-column injection, stopped-flow injection, pressure pulse-driven stopped-flow injection (PSI), groove injection, split injection, heart-cut injection, and the moving injection technique (MIT). Of the injection techniques, only the split injector, MIT and PSI approaches can introduce subnanoliter sample volumes accu-... 

The separation of various arsenic species is a good example of the application of ICP-AES detection to anion chromatography [26]. A microbore colunm 10 cm X 1.7 mm I.D. was used with a low flow rate (<100 pL/min). The column was packed with a low-capacity anion-exchange material (0.05 mequiv/g and solution containing 5 mM ammonium carbonate and 5 mM ammonium bicarbonate at pH 8.6 served as the mobile phase. The column hardware was connected directly to the inlet of the DIN-ICP-AES via a short length of 0.3 mm I.D. PEEK tubing. 

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