Microbial cultures hosts

There are several approaches that can be taken to supply material for pharmaceutical evaluation. Two of these, invertebrate cell culture and fermentation of associated microorganisms, begin with determining the biogenetic origin of the compound. There are numerous reports of metabolite localization, primarily in sponges, in which production of a bioactive metabolite has been inferred or demonstrated to be localized either in a host invertebrate cell or in microbial associates which include cyanobacteria and heterotrophic bacteria and fungi.72-73... 

Host-mediated assays Mouse or rat Uses microbial indicator organisms or cell cultures As in indicator test system 1 mo M M M NA... 

A new dibenzodiazepine alkaloid, diazepinomicin 265, was isolated from the culture of a marine actinomycete of the genus Micromonospora Compound 265 showed modest anti-microbial activity against selected Gram-positive bacteria with MICs of about 32 pg mL". The method of expression the biosynthetic pathway genes in transformed host cells, and the novel polynucleotide sequences and their encoded proteins involved in the biosynthesis of 265 were discussed. Derivatives of 268 were prepared. ... 

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