Methylation of metallated

Thayer, J. S. Brinkman, F. E. (1982). The biological methylation of metals and metalloids. Advances in Organometallic Chemistry, 20, 313-57. 

The formation of methoxide complexes was studied by measuring the pH in titrations, with methylate, of the chlorides and methylates of metals, in absolute methanol. The pH determinations were carried out with Pt/H or Pd/Hj electrodes, which were found to respond rapidly, reproducibly and strictly proportional to the logarithms of the concentrations of solvated protons. The problem of changing activity coefficients and ion-pair formation were... 

Many models for vitamin B12, e.g. Schrauzer s cobaloximes [125], have been developed. Methylcobalamin is the agent for the methylation of metals e.g. to [HjC-Hg] (Scheme 1.16). It is interesting to note that bacteria (mercury lyases) can cleave the mercury carbon bond. This type of cleavage together with the reduction of organometallic compounds by biocatalysts has been reviewed by Ryabov [13]. 

The non-biodegradable water soluble heavy metals are either oxidized or reduced by the microorganisms and produce less soluble species. The less soluble form of these metals which are formed due to microbial reactions are adsorbed or precipitated/co-precipitated on the surface of the adsorbent and the extra cellular protein of the microorganisms in the biolayer (Srivastava and Majumder, 2008 Vails and Lorenzo, 2002). The methylation of metals is also another important route for bioremediation of heavy metals in water (White et al, 1997). Though the microbial action on metal ion transformation is still a matter of research, it is assumed that there are two paths. In one path oxidation or reduction of heavy metal ions takes place by extra cellular enzymes where the metal ions do not enter into the bacterial cell. In the other path the metal ions are transported into the microbial cells by trans-membrane proteins and are converted to other less soluble forms by metabolic actions of enzymes in the cells followed by subsequent excretion from the cells, yet both the paths are plasmid mediated (Vails and Lorenzo, 2002). Whether the microbial action on a metal ion is performed by only one path or by both the paths is a matter of research. 

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