Method Selection and Optimization

In the following sections, method selection and optimization of separations for some of the CSPs described in Table 2 are discussed. 

Method selection and optimization of separations might require different conditions depending on the selector and the stationary phase. The following paragraphs give an overview of the main features of commonly employed CSPs. It is recommended that before using a new CSP the information supplied by the manufacturer should always be studied carefully in order to avoid inadvertent destruction of the CSP by selecting an unsuitable eluent 

Natural products such as cellulose and amylose have proved to be of great versatility for enantiomer separations. Though poor chiral selectors in their native state, they become highly effective when their hydroxyl functions are derivatized, particularly with aromatic moieties through ester or carbamate linkages, and they are suitably immobilized on silica supports [11]. 

These materials are composed of small chiral imits that are regularly repeated along the polymeric chain, hence the density of active sites capable of chiral recognition is very high, and this results in a high loading capacity. MicrocrystaUine cellulose triacetate and cellulose tribenzoate have, in some instances, been used for chiral separations as neat, non-coated particles. However, they are inconvenient to pack, are of limited chemical stability, and show low efficiency. 

Eluent or eluent mixture (composition In vol%) Chiralpak AD chiralpak AS Chiralcel OD Chiralcel OJ 

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