Method development considerations

The following physico-chemical properties of the analyte(s) are important in method development considerations vapor pressure, ultraviolet (UV) absorption spectrum, solubility in water and in solvents, dissociation constant(s), n-octanol/water partition coefficient, stability vs hydrolysis and possible thermal, photo- or chemical degradation. These valuable data enable the analytical chemist to develop the most promising analytical approach, drawing from the literature and from his or her experience with related analytical problems, as exemplified below. Gas chromatography (GC) methods, for example, require a measurable vapor pressure and a certain thermal stability as the analytes move as vaporized molecules within the mobile phase. On the other hand, compounds that have a high vapor pressure will require careful extract concentration by evaporation of volatile solvents. 

N. R. Srinivas, Simultaneous chiral analyses of multiple analytes Case studies, implications and method development considerations, Biomed. Chromatogr. 18 (2004), 759-784. 

During method development, considerable work may be required to make a judicious choice for the lower limit of quantification (LLOQ). To obtain an initial... 

PCSL Solvent Bonding Method Development Considerations... 

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