Methionine crystal structure

This pyridoxal phosphate-requiring enzyme has been studied in several bacteria and X-ray crystal structures are available.35 The coryneform bacterium, Brevi-bacterium linens, is common on the surface of several cheeses, including Limburger and those of the Trappist type. The methionine y-lyase of this organism has been purified to homogeneity36 and the relevant gene, mgl (from MGL, abbreviation for methionine y-lyase) has been cloned and analyzed.37... 

STD-NMR experiments applied to the MDWNMHAA/Eab complex allowed the identification of the peptide residues in direct contact with the antibody, and a comparison with the crystal structure [100]. Strong enhancements of all the ring protons of Trp P3 were observed, consistent with the extensive contacts of this residue with the antibody. In addition, the /3-CH2 protons of Asn P4 were enhanced, and the methyl groups of Ala P7 and Ala P8. Enhancements were detected for both methionine g-CH3 groups, with... 

Crystal structures of methyltransferases suggest that S-adenosyl-L-methionine binds in an extended conforma-... 

Freeman, H. C., and Golomb, M. L. (1970). Model compounds for metal-protein interaction Crystal structure of three platinum(Il) complexes of l- and DL-methionine and glycyl-L-methionine. Chem. Commun. pp. 1523-1524. 

So far only a few dozen organofluorine compounds have been isolated from living organisms, for example fluoroacetic acid, 4-fluorothreonine and rw-fluoro-oleic acid [244-246], The reason that nature has not invested in fluorine chemistry could be a combination of low availability of water-dissolved fluoride in the environment due to its tendency to form insoluble fluoride salts, and the low reactivity of water-solvated fluoride ion. However, in 2002, O Hagan and collaborators [247] published the discovery of a biochemical fluorination reaction in a bacterial protein extract from Streptomyces cattleya converting S-adenosyl-L-methionine (SAM) to 5 -fluoro-5 deoxyadenosine (5 -FDA). The same protein extract contained also the necessary enzymatic activity to convert 5 -FDA into fluoroacetic acid. In 2004, the same authors published the crystal structure of the enzyme and demonstrated a nucleophilic mechanism of fluorination [248,249]. 

Figure 7. Crystal structures of the ternary complexes of [Yb.la]3+ with (

X Cheng, S Kumar, J Posfai, JW Pflugrath, RJ Roberts. Crystal structure of the Hhal DNA methyltransferase complexed with S-adenosyl-L-methionine. Cell 74 299-307, 1993. 

The X REL1 crystal structure, which consists of residues 52-316 corresponding to the N-terminal domain, was resolved using the SeMet multiple-wavelength anomalous dispersion method (9). The selenomethionines used for this method should be replaced with methionines to obtain the original form of the protein for simulations (see Note 2). 

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