Metabolism studies, HPLC pesticide

We believe that this symposium on Recent Advances in Pesticide Analytical Methodology fulfills this plea. High-performance liquid chromatography (HPLC) has made the greatest advances. Chapters on HPLC cover subjects on metabolism studies automation of HPLC evaluation of LC columns the effect of the mobile phase on reversed-phase chromatography the electrochemical or amperometric detector and fluorogenic detection. 

There are many applications in the studies of the metabolism of drugs, pesticides, etc., in which radioactive samples are employed to enable the compounds of interest to be detected at very low concentrations. The important current trends in HPLC are the use of super critical fluids as eluants and the coupling of HPLC with NMR, MS, or FTIR. 

A study has been carried out on the determination of triazine and carbamate pesticides and metabolities in seawater by HPLC with photodiode-array detection [393]. 

The recent development of commercial HPLC systems has provided a powerful instrumentation for the separation, characterization, identification, and quantitation of minute amounts of essential dietary components (68,69). Developments in hardware and packings for HPLC have overcome the problems of nonreproducible behavior and low efficiency separations previously associated with column chromatography (70). HPLC has already been applied to the quantitative analysis of analgesics, pesticides, and fat-soluble vitamins with precision and accuracy and a minimum of sample clean-up. Such instrumentation provides a rapid, accurate, and sensitive technique for the separation and analysis of subnanomole quantities of a wide range of complex high-molecular-weight, nonvolatile, thermally labile, compounds that are vital for metabolic and nutritional studies. 

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