Metabolic resveratrol

Urpi-Sarda M, Zamora-Ros R, Lamuela-Raventos R, Cherubini A, Jauregui O, de la Torre R, Covas MI, Estruch R, Jaeger W and Andres-Lacueva C. 2007. HPLC-tandem mass spectrometric method to characterize resveratrol metabolism in humans. Clin Chem 53(2) 292-299. 

J. W. Hirschelman, W. H. et ah Increasing the throughput and productivity of Caco-2 cell permeability assays using liquid chromatography-mass spectrometry application to resveratrol absorption and metabolism. Comb Chem High Throughput Screen 2003, 6, 757-767. 

Palsamy P, Subramanian S. (2009) Modulatory effects of resveratrol on attenuating the key enzymes activities of carbohydrate metabolism in strep-tozotocin-nicotinamide-induced diabetic rats. Chem Biol Interact 179 356-362. 

Resveratrol improves mitochondrial function and protects against metabolic disease by activating SIRTl and PGC-lalpha. Cell, 127, 1109-1122. 

Kaneider, N.C., Mosheimer, B., Reinisch, N., Patsch, J.R., and Wiedermann, C.J., Inhibition of thrombin-induced signaling by resveratrol and quercetin effects on adenosine nucleotide metabolism in endothelial cells and platelet-neutrophil interactions, Thromb. Res., 114, 185, 2004. 

While consumption of products rich in resveratrol appears to be beneficial for the reasons discussed above, little is known about resveratrol bioavailability in humans and animals. Emilia et al. (1999) developed an analytical method to measure stilbene present in blood. Resveratrol administered orally to rats was detected in plasma. Excellent HPLC-based separation of tram-resveratrol from other compounds in the blood was achieved, allowing a rapid analysis of the sample for absorption, distribution, and metabolism studies. 

Several studies have investigated the absorption, transport, and metabolism of resveratrol in vitro and ex vivo. They are summarized in Table 13.1. 

Table 13.1 Metabolism of Resveratrol in Vitro and Ex Vivo Models 2...

The metabolites of [3H] /ra ,v-resveratrol detected in tissues and plasma were also investigated. In kidney, liver, heart, lungs, brain, and plasma (2-h samples), the only metabolite found was resveratrol glucuronide. Glucuronides in plasma and kidney disappeared completely at 18 h. In lungs, liver, heart, and brain, the main detected metabolite at 18 h was the /ra ,v-resveratrol (Table 13.2). This study provided data on the metabolic fate of resveratrol. While glucuronides were predominant in plasma and tissues at the earlier times, the aglycone represented the main form at later times [El-Mohsen et al., 2006]. 

Various studies have focused on the metabolism of piceid, the glucoside of resveratrol. These studies are of great interest due to the higher amount of piceid compared to resveratrol in food. Therefore, bioavailability studies of this compound are required. In vitro studies have already observed the absorption of piceid in the enterocytes, but in vivo studies in animal models are still scarce. 

Figure 13.3 Pathways of resveratrol absorption, distribution, metabolism, and excretion.

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