Membranes intermediate

Y van Heijenoort, M Gomez, M Derrien, J Ayala, J van Heijenoort. Membrane intermediates in the peptidoglycan metabolism of Escherichia coli possible roles ofPBPlb and PBP3. J Bacterid 174 3549-3557, 1992. 

At the plasma membrane. Intermediate filaments are attached by adapter proteins to specialized cell junctions called... 

MAPs are classified into two groups on the basis of their function. One group stabilizes microtubules. The stuc-ture of a stabilizing MAP consists of two domains—a basic microtubule-binding domain and an acidic projection domain. In the electron microscope, the projection domain appears as a filamentous arm that extends from the wall of the microtubule. This arm can bind to membranes, intermediate... 

Liposomes loaded with various drugs can incorporate these drugs in a variety of fashions water-soluble drugs are entrapped into the liposomal inner aqueous space (and, in case of multilam-mellar liposomes, into the aqueous space between bilayers), while less soluble drugs may be incorporated into the phospholipid membrane. Intermediate cases also exist. 

Section 26 4 Phospholipids are intermediates in the biosynthesis of triacylglycerols from fatty acids and are the principal constituents of the lipid bilayer component of cell membranes... 

Both PSI and PSII are necessary for photosynthesis, but the systems do not operate in the implied temporal sequence. There is also considerable pooling of electrons in intermediates between the two photosystems, and the indicated photoacts seldom occur in unison. The terms PSI and PSII have come to represent two distinct, but interacting reaction centers in photosynthetic membranes (36,37) the two centers are considered in combination with the proteins and electron-transfer processes specific to the separate centers. 

Most of the naturally-occurring pyrazine hydroxamic acids appear to be derived from valine, leucine and isoleucine, and biosynthetic studies by MacDonald and coworkers (61JBC(236)512, 62JBC(237)1977, 65JBC(240)1692) indicate that these amino acids are incorporated. However, it would seem that the logical intermediates, viz. the 2,5-dioxopiperazines such as (111) and (112), are not always incorporated. This does not rule out their intermediacy, as there may be problems such as low solubility or membrane permeability which prevent their efficient incorporation. An exception to these results was reported for pulcherrimic acid (113) (65BJ(96)533), which has been shown to be derived from cyclo-L-leu-L-leu which serves as an efficient precursor. 

Modules Eveiy module design used in other membrane operations has been tried in peivaporation. One unique requirement is for low hydraulic resistance on the permeate side, since permeate pressure is veiy low (O.I-I Pa). The rule for near-vacuum operation is the bigger the channel, the better the transport. Another unique need is for neat input. The heat of evaporation comes from the liquid, and intermediate heating is usually necessary. Of course economy is always a factor. Plate-and-frame construc tion was the first to be used in large installations, and it continues to be quite important. Some smaller plants use spiral-wound modules, and some membranes can be made as capiUaiy bundles. The capillaiy device with the feed on... 

Fibrous or particulate filters are not important anymore because membrane filters are relatively compac t and perform veiy well. For filtration by straining, there is an intermediate air velocity at which filtration efficiency is a minimum because different collec tion mechanisms predominate at different ranges of velocity. At low velocities, diffusional and elec trostatic forces on the particle are important, and increased velocity shortens the time for them to operate. At high velocities, inertial forces that increase with air velocity come into play below a certain air velocity, their effect on collection is zero. Surges or brief power failures could change velocity and collection efficiency. 

FIGURE 18.5 Schematic representation of types of multienzyme systems carrying out a metabolic pathway (a) Physically separate, soluble enzymes with diffusing intermediates, (b) A multienzyme complex. Substrate enters the complex, becomes covalently bound and then sequentially modified by enzymes Ei to E5 before product is released. No intermediates are free to diffuse away, (c) A membrane-bound multienzyme system. 

All these intermediates except for cytochrome c are membrane-associated (either in the mitochondrial inner membrane of eukaryotes or in the plasma membrane of prokaryotes). All three types of proteins involved in this chain— flavoproteins, cytochromes, and iron-sulfur proteins—possess electron-transferring prosthetic groups. 

Treatment of 2,6-dimethylaniline (121) with phosgene and triethylamine affords the corre-S]ionding isocyanate (122). Condensation of that reactive intermediate with N-isopropylpropyl-cne-1,3-diamine leads to formation of urea 123. This product, recainam (123), acts as membrane Stabilizing agent and thus exhibits both local anesthetic and antiarrhythmic activity [30]. 

Cholesterol is a widely distributed sterol found free or esterified to fatty acids. It is an important intermediate in the biosynthesis of steroid hormones and the principal component of cell plasma membranes and the membranes of intracellular organelles. 

COPII vesicles are transport intermediates from the endoplasmic reticulum. The process is driven by recruitment of the soluble proteins that form the coat structure called COPII from the cytoplasm to the membrane. 

---