Media incubation conditions selection

The test was carried out in 500 ml cylindrical vessels containing 100 ml of a mineral based culture medium, inoculated with a selected PVA-degrading mixed bacterial culture. Incubation was carried out under static conditions at 20 C. 

We propose that conditions altering the availability of NAD may be signaled to chromatin by the intermediacy of poly(ADP-ribose) (Fig. 2). The question is whether this signal operates imder physiological conditions. Some reports have provided direct evidence that NAD- depletion forced upon cells by incubation in nicotinamide-free medium, arrests poly(ADP-ribose) synthesis (5) and drastically alters chromatin functions (5-7). For example, DNA repair in response to a chemical carcinogen was arrested under these conditions, but could be reestablished with nicotinamide, which restored normal intracellular NAD- and poly(ADP-ribose) levels (5). Similarly, myoblast differentiation was reversibly inhibited either by nicotinamide starvation or by selective inhibition of poly(ADP-ribose) polymerase (7). These results illustrate that nutritional manipulation of... 

Determination of micro-organism content. To estimate the micro-organism content of a sampled aliquot requires selecting subculture conditions (medium, temperature, dilution, incubation temperature and time) appropriate for unrestricted growth of the preservative-stressed micro-organisms, and adequate inactivation of the preservative system carried over in the sample aliquot. 

RBC were conditioned as already described ". In most cases, 0.8 ml of packed RBC collected after the third washing were suspended in 1.2 ml of the selected experimental medium, namely autologous plasma, autologous serum, albumin buffer or Tris buffer, resulting in a 40% hematocrit. Tris buffer was used to wash the RBC to be suspended in Tris buffer, plasma or serum. RBC to be suspended in 40g/l albumin buffer were washed with PBS. The suspensions were incubated at 37°C for 10 min. Aliquots of 0.2 ml of the solutions to be tested comparatively, namely Tris buffer, 2mM or lOmM polycation, were then added to RBC suspensions. The final mixtures were further incubated at 37°C for 15 min and then centrifuged at 1910 x g for 10... 

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