McCleary method

Recently, the most commonly used in vitro methods were extensively evaluated and a simplified version was proposed (McCleary method). Here, samples are treated with an amyloglucosidase-pancreatic amylase mixture only and the insoluble residue, after washing with ethanol, is dispersed with KOH, followed by the amyloglu-cosidase step to yield glucose. This protocol has been accepted by AOAC International (AOAC method 2002.02) and the American Association of Cereal Chemists (AACC method 32-40) (Table 4). 

The Megazyme procedure requires the company s Total Starch Assay Kit, which contains most of the necessary enzymes and chemicals. This procedure was approved by the American Association of Cereal Chemists (AACC Method 76-13 AACC, 1976) and the Association of Official Analytical Chemists (AOAC Method 996.11) following an extensive collaborative AACC/AOAC interlaboratory evaluation (McCleary et al., 1997b). The procedure is simpler than a previously reported method (AACC, Method 76-12). The procedure by Holm et al. (1986) described in the Alternate Protocol uses enzymes and chemicals purchased individually. 

McCleary, B.V., Gibson, T.S., and Mugford, D.C. 1997a. Collaborative evaluation of a simplified assay for total starch in cereal products (AACC Method 76-13). Cereal Foods World 42 476-480. 

The concentration of short-chain fructooligosaccharides in foods is of interest in that they comprise part of the total dietary fiber. The standard Asssociation of Official Analytical Chemists (AOAC) method for dietary fiber does not measure short-chain fructooligosaccharides due to their ethanol solubility however, several alternative methods have been developed (Hoebregs, 1997 McCleary et al., 2000 Ouame et al., 1997 Simonovska, 2000 Zuleta and Sambucetti, 2001). 

McCleary, B.V., Murphy, A., and Mugford, D.C., Measurement of total fructan in foods by enzymatic/spec-trophotometric method collaborative study, J. Assoc. Off. Anal. Chem. Int., 83, 356-364, 2000. 

Lemos MANDA, Pombeiro AJL (1993) A comparative study of numerical methods for cyclic voltammetry digital simulation of an electrochemical process with a coupled chemical reaction. In Pombeiro AJL, McCleary JA (eds) Molecular electrochemistry of inorganic, bioinorganic and organometallic compounds. Kluwer Academic, Dordrecht, pp 477-482... 

Dragoin, W.B., G.E. McCleary P.A. McCleary. 1971. A comparison of two methods of measuring conditioned taste aversions. Behav. Res. Methods and Instr. 3 309-310. 

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