Matrix metalloproteinase sensitive hydrogels

Lutolf MP, Lauer-Fields JL, Schmoekel HG et al (2003) Synthetic matrix metalloproteinase-sensitive hydrogels for the conduction of tissue regeneration Engineering cell-invasion characteristics. Proc Natl Acad Sci 100 5413-5418... 

Lutolf, M.P. et al. Synthetic matrix metalloproteinase-sensitive hydrogels for the condudion of tissue regeneration engineering cell-invasion characteristics, Proc. Natl Acad. Sci USA, 100, 5413, 2003. 

Lutolf, M. R, Lauer-Fields, J. L., Schmoekel, H. G., Metters, A.T., Weber, F. E., Fields, G. B. and Hubbell, J. A. (2003a). Synthetic matrix metalloproteinase-sensitive hydrogels for the conduction of tissue regeneration Engineering cell-invasion characteristics. Proceedings of the National Academy of Sciences of the United States of America. 100,5413-5418. 

Park, J., Lim, E., Back, S., Na, H., Park, Y., Sun, K., 2010. Nerve regeneration following spinal cord injury using matrix metalloproteinase-sensitive, hyaluroiuc acid-based hiomimetic hydrogel scaffold containing brain-derived neurotrophic factor. J. Biomed. Mater. Res. A 93 (3), 1091-1099. 

Park, Y, Lutolf, M.P., Hubbell, J.A. et al. 2004. Bovine primary chondrocyte culture in synthetic matrix metalloproteinase-sensitive polyfethylene glycol)-based hydrogels as a scaffold for cartilage repair. Tissue Eng 10(3-4) 515-22. 

The introduction of enzyme-sensitive cross-links in an otherwise nonenzyme-responsive polymer such as PEG is frequently used to prepare enzyme-responsive polymer hydrogels and polymer particles. Because of their versatility and natural predisposition as enzyme substrates, short peptide sequences are almost exclusively used as cross-linkers, although dex-tran has also been used (Klinger et al., 2012). They can be readily changed to respond to a variety of proteases such as matrix metalloproteinases,plasmin or trypsin (Lutolf et al., 2003a Yang et al, 2010). In most cases, the peptides have to be modified at the termini to introduce reactive groups that are able to react with the polymer. 

Turturro, M., Christenson, M., Larson, , and Papavasihou, G. (2012). Matrix metalloproteinase (MMP) sensitive PEG diacrylate (PEGDA) hydrogels with spatial variations in matrix properties direct vascular cell invasion. Journal of Tissue Engineering and Regenerative Medicine, 6,302. 

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