Matrix-assisted laser proteomic profiling

Many diseases are characterized by the expression of specific proteins1 in some cases, malignant cells yield unique protein profiles when total cellular protein extracts are analyzed by proteomic methods such as two-dimensional gel electrophoresis or matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS).2 High-throughput proteomic studies may be useful to differentiate normal cells from cancer cells, to identify and define the use of biomarkers for specific cancers, and to characterize the clinical course of disease. Proteomics can also be used to isolate and characterize potential drug targets and to evaluate the efficacy of treatments. 

Matrix-assisted laser desorption ionization (MALDI) and surface-enhanced laser desorption ionization (SELDI) have been used online with TOF-MS for protein differential profiles of intact or hydrolyzed biological matrices in proteomics. The potential use of affinity chips, grafted with specific Ab towards the drug compound for MALDI or SELDI, will bring sensitive and selective tools for macromolecules. Specific Ab towards either the intact protein or several signature peptides... 

Mitchell, B.L. et al.. Evaluation of matrix-assisted laser desorption/ionization-time of flight mass spectrometry proteomic profiling Identification of alpha 2-HS glycoprotein B-chain as a biomarker of diet, Proteomics, 5, 2238, 2005. 

Hettick JM, Kashon ML, Simpson JR Siegel PD, Mazurek GH, Weissman DN. Proteomic profiling of intact mycobacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal Chem. 2004 76(19) 5769-76. doi 10.1021/ac049410m. 

Diamandis, E.R, Serum proteomic profiling by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry for cancer diagnosis next steps. Cancer Res., 66,5540-5541 (2006). Engwegen, J.Y., Gast, M.C., Schellens, J.H. and Beijnen, J.H., Clinical proteomics searching for better tumour markers with SELDI-TOE mass spectrometry. Trends Pharmacol. Sci., 27, 251-259 (2006). 

Chaurand, R, DaGue, B.B., Pearsall, R.S., Threadgill, D.W. and Caprioli, R.M., Profiling proteins from azoxymethane-induced colon tumors at the molecular level by matrix-assisted laser desorption/ionization mass spectrometry. Proteomics, 1, 1320-1326 (2001). 

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