Protein sensing

Applications of Molecular Rotors in Protein Sensing and Sensing of Other... 

How do the "gates" to ion channels open Presumably some part of the channel protein senses the change in potential and undergoes an appropriate alteration in conformation that opens the gate.434 The current carried by the ions flowing out through a small... 

Keitzmann, T., H. Schmidt, K. Unthan-Feschner, I. Probst, and K. Jungermann (1993). A ferro-heme protein senses oxygen levels which modulate the glucagon dependent activation of the PEPCK gene in rat hepatocyte cultures. Biochem. Biophys. Res. Comm. 195 792-798. 

Materials based on reversibly covalently bound constituents could also be applied in the construction of constitutionally dynamic systems [117]. For example, the glycodynamers could be exploited as adaptive layers for selective protein sensing [118] or adaptive nucleic acid [119, 120] displays could be exploited for DNA sensing applications. These sensing interfaces could be pre-conditioned and fixed for optimal sensing of the choice analyte using the principles already established in DCC [1]. 

The crystal structure of a channel clarified the basis for selection of ions that can pass through the open channel pore and the mechanism by which the channel proteins sense changes in transmembrane voltage that control the open or closed conformational states of the channel (5). Thus, investigations of ion channel proteins employ fundamental physics to study the function of biologically critical proteins. 

This class of compounds is a convenient starting material en route to asymmetrically substituted ferrocenoyl derivatives, which may potentially have applications in proteomics and protein sensing. An unusual procedure was reported in 1996 by Erker and co-workers leading to the formation of Fc-Val-OMe (Val = Valine) 22, Fc[Val-OMe]2 12 (Scheme 12.4) from two equivalents of the Li-salt of a Cp-Val = OMe conjugate with anhydrous FeCl2 in tetrahydrofuran (THF) 43... 

How do these proteins sense changes in membrane potential ... 

Protein sensing involves detection of the protein component of the pathogenic bacteria, viruses, and individnal toxins. It requires minimal sample preparation. Specificity is achieved by using antibodies, receptors, or aptamers raised or selected to specifically bind surface proteins or whole bacteria, bacterial spores, viral particles, or individual toxins. 

Sexton, L.T., Mukaibo, H., Katira, R, Hess, H., Sherrih, S.A., Home, L.R, Martin, C.R. An adsorption-based model for pulse duration in resistive-pulse protein sensing. J. Am. Chem. Soc. 132, 6755-6763, 2010. 

Insertion of a globular protein sensing element within an elastic model protein chain results in a force-extension profile that contains the unfolding peak for the single globular protein. 

Nanomaterials in Protein Sensing Devices 1.3.1 Nanomaterials in Electrochemical Immunoassays... 

Tkac, J., Davis, J.J. Label-free field effect protein sensing. In Davis, J.J. (ed.) Engineering the Bioelectronic Interface, pp. 193-224. Royal Society of Chemistry, UK (2009)... 

FIGURE 4.2.3 Schematic illustration of protein sensing by direct ELISA (a) and sandwich ELISA (b). 

The coUoid-probe measurements may be most pertinent to the interpretation of interfacial interactions between these coatings and large, usually overall negatively charged proteins that would not be able to penetrate, by diffusion, into the steric barrier layer but are likely to be able to sense the EDL force. If such proteins sense an attractive EDL force originating from beneath the PEG layer, as is the case for lower PEG graft densities, they... 

For example, one important enzyme (pyruvate kinase) that breaks down sugar works differently with electrons/hydrogens on its sulfurs. So does an enzyme that sends proteins to the trash. A third example is how one bacterial protein senses bleach with a sulfur and adopts a new shape when bleach is around, alerting the rest of the cell. 

Bilitewski, U. (2006) Protein-sensing assay formats and devices. Anal. Chim. Acta, 568, 232-247. 

Zhang, Z., Wang, Y, Yan, R, Peng, D., Ma, Z. (2011). Photosensitive azopolymer brushes via atom transfer radical polymerization for protein sensing. Chinese Journal of Chemistry, 29, 153-158. http //dx.doi.Org/10.1002/cjoc.201190044. 

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