Mannose ligand binding

E. A. L. Biessen, F. Noorman, M. E. van Teijlingen, J. Kuiper, M. Barrett-Bergshoeff, M. K. Bijsterbosch, D. C. Rijken, and T. J. C. Van Berkel, Lysine-based cluster mannosides that inhibit ligand binding to the human mannose receptor at nanomolar concentration, J. Biol. Chem., 271 (1996) 28024—28030. 

Fig. 3. Concanavaline-A-induced, ligand-binding assay of various liposomal formulations. Concanavalin A solution (lOOgg/mL) was added to 0.2 mL of diluted liposomes formulations and absorbance at 550 nm was measured as a function of time. The data were subtracted from the blank experiment conducted without an addition of concanavalin A. Binding of the terminal mannose residues of the mannan to the ConA causes agglutination of the complex in solution resulting in an increase in turbidity. However, plain liposomes did prevent the binding of Con A as evidenced by the lower turbidity. The accessibility of the mannan to the liposomes is critical for in vivo cell binding...

Figure 4 The mannose binding protein A (MPB-A) binding site featuring van der Waals contacts with the mannose ligand.

K. Drickamer, M.-C. Shao, Y.C. Lee, Ligand-binding characteristics of rat serum-type mannose-binding protein (MBP-A), J. Biol. Chem. 1991, 266, 4810-4815. 

The triple-helical structnre has also been identified in a range of other proteins, such as macrophage scavenger receptor, mannose-binding protein, and Clq, most of which play a role in host defence mechanisms. In these cases, the triplehelical domain often exhibits ligand binding, which is key to the function of the protein (14). 

Recently, Schmidt and coworkers introduced a novel assay to determine binding affinities and IC50 values by mimicking more closely cell-cell contacts and their inhibition. They synthesized soft colloidal particles (SCPs) functionalized with mannose ligands and incubated these microgels on a... 

In 2008, Pohl et al. [12f] described an innovative technology based on fluorous-based carbohydrate microarrays applied to demonstrate that both diastereomers of glycero-D-mannoheptoses fotmd in bacteria bind to ConA. These results showed that this plant lectin can accept modifications at the C-6 position of its natural mannose ligand. 

York, S. J., L. S. Arneson, W. T. Gregory, N. M. Dahms, and S. Komfeld. 1999. The rate of internalization of the mannose 6-phosphate/insulin-like growth factor II receptor is enhanced by multivalent ligand binding. J Biol Chem. 274 (2) M- 1. ... 

Wendland, M., A. Waheed, B. Schmidt, A. Hiile, G. Nagel, K. von Figura, and R. Pohlmann. 1991. Glycosylation of the Mr 46,000 mannose 6-phosphate receptor. Effect on ligand binding, stability, and conformation. J Biol Chem. 266 f7j 4598-4604. 

Li, M. M., and G. W. Jourdian. 1991. Isolation and characterization of the two glycosylation isoforms of low molecular weight mannose 6-phosphate receptor from bovine testis. Effect of carbohydrate components on ligand binding. J Biol Chem. 266 ( 26,1 17621-17630. 

Marron-Terada, P. G., M. A, Brzycki-Wessell, and N. M. Dahms. 1998. The two mannose 6-phosphate binding sites of the insulin-like growth factor-II/mannose 6-phosphate receptor display different ligand binding properties, J Biol Chem. 273 (35)-.2235S-22366. 

The remainder of this review will focus on the collectins, which like the macrophage mannose receptor, bind to carbohydrate ligands on bacterial, fungal and parasitic cell walls and constitute an integral part of the mammalian innate inunune response. 

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