Mannose labeled

Fig. 4.5.6 Lipid-linked oligosaccharides (LLO) isolated from different CDG-I patients. Fibroblasts from a control and different CDG-I patients (CDG-lc, CDG-ld, CDG-lg, CDG-li) were metabolically labelled with 2-[3H]mannose for 30 min. [3H]oligosaccharides were released from LLO by mild acid hydrolysis and size-fractionated by HPLC. M5, M7, M9 and G3 refer to the positions of GlcNAc2Man5, GlcNAc2Man7, GlcNAc2Man9 and GlcNAc2Man9Glc3, respectively. The arrow marks shortened [2-3H]mannose-labelled oligosaccharides accumulating in the case of very early CDG-I types like CDG-li, which are often hard to detect by standard LLO-HPLC analysis...

Mannose Wt/lysines Controlled aggregation of mannose-labeled CPMV via interaction with concanavalin A [102]... 

Figure 3. Distribution of carbohydrate and in fractions from Bio-Gel P-2 following acetolysis of -mannose-labeled peptidophosphogalactomannan. The re-tion was conducted as described (12) and the products following acetolysis were fractionated on a column of Bio-Gel P-2. The...

Anzai, J. Kobayashi, Y. Nakamura, N. Alternate deposition of eoneanavalin A and mannose-labeled enzymes on a solid surface to prepare catalytically active enzyme thin films. J. Chem. Soc., Perkin Trans. 2 1998, 461. 

Kobayashi, Y. Hoshi, T. Anzai, J. Glucose and lactate biosensors prepared by a layer-by-layer deposition of eoneanavalin A and mannose-labeled enzymes electrochemical response in the presence of electron mediators. Chem. Pharm. Bull. 2001, 49, 755. 

Transfer from dolichol diphosphate-mannose labelled oligosaccharide to protein has been studied with enzymes from liver (Behrens et al., 1973), hen oviduct (Lucas et al., 1975) and myeloma cells (Hsu et al., 1974). The results were similar to those described before in that not one but many proteins seem to become labelled. In the case of hen oviduct which produces mainly ovalbumin only less than 10% of the label was recovered in ovalbumin (Lucas et al., 1975). For reviews on the subject see Behrens (1974), Lennarz (1975), Parodi Leloir (1975) and Waechter Lennarz (1976). 

Problem 25,13 Draw /3-o-galactopyranose and jS-D-mannopyranose in their more stable chair conformations. Label each ring substituent as either axial or equatorial. Which would you expect to be more stable, galactose or mannose ... 

D. L. Jones and P. R. Darrah, Re-sorption of organic compounds by roots of Zea mays L. and its consequences in the rhizosphere I. Re-sorption of C labelled glucose, mannose and citric acid, Plant and Soil 143 259 (1992). 

Formaldehyde or glutaraldehyde Labeled mannose Polysaccharide Considerable 20% loss of radioactivity with either fixative Vanha-Pertulla and Grimley (22)... 

Figure 14.8 (A) Screening of a glycopeptide library using a fluorescent-labeled lectin and ligands bound to PEGA beads. The acbve compounds are analysed by mass spectrometry. (B) FITC-labeled lectin binding to resin bound mannose could be inhibited by soluble glycopeptides obtained from library screen. Percent inhibition was quantified by recording of lectin fluorescence. Only every second well of the microtiter plate was used and nonfluorescent beads indicated good inhibitors.44...

T. Ido, C.N. Wan, V. Casella, J.S. Fowler, A.P. Wolf, M. Reivich, D.E. Kuhl, Labeled 2-deoxy-D-glucose analogs—F-18-labeled 2-deoxy-2-fluoro- o-glucose, 2-deoxy-2-fluoro- D-mannose and C-14-2-deoxy-2-fluoro- o-glucose, J. Label. Compds Radiopharm. 14 (1978) 175-183. 

Measurements of the quantities of glycolipids inserted into the membrane have also been reported by a technique based on the use of C-labeled lipid anchors. In this method, the carbohydrate (a-o-Man) was covalently coupled to the anchor at the surface of a pre-formed vesicle. Indeed, the liposome structure was shown to remain intact in the treatment. Nevertheless, the measurement of the incorporated mannose was performed after separation of bound and unbound material by centrifugation. The yields of coupling were shown to increase with the increase of the initial mannose/ C-anchor ratio, but non covalent insertions were displayed at high initial mannose concentrations. Therefore, the aforementioned method was not as accurate as could have been expected for the use of radioactive materials [142]. Radiolabeled phospholipids were also used for such determinations thus the amounts of glycosphingolipids incorporated into liposomes were quantified by the use of H-phospholipids whereas the amounts of glycolipids were determined by a sphingosine assay [143]. 

The nascent, 3-carbon anion liberated from the reducing end of D-mannose-i-14C can participate directly in an aldol reaction, to form hexose-3-14C, but it can only afford hexose-i-14C after isomeriz-ing. Thus, the mixture of radioactive hexoses would be mainly C-3-labeled, and would give radioactive a -D-glucosaccharinic acid labeled principally at C-2 this agrees with the experimental result. The formation of the anion (81) could well enhance this effect by... 

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