Mannan derivatives

Several pharmaceutical activities of nucleic acid analogs such as poly(VAd) have been studied in vitro and in cell-free systems [19]. It was expected that the present polymers would be effectively transferred into phagocytes by encapsulating in polysaccharide-coated liposomes and would show increased pharmaceutical activities similar to poly(maleic acid-a/l-2-cyclohexyl-l, 3-dioxap-5-ene) (MA-CDA) [68]. The activation of human neutrophils by poly(VAd) was evaluated by monitoring the in vitro superoxide anion production from activated human neutrophils. Shown in Table 15 is the superoxide liberated from human neutrophils (1 x 106 cells/ml) activated by poly(VAd) (0.5 mg/ml) as a function of time. Poly(VAd) encapsulated in mannan derivative-coated liposomes showed a... 

Fig. 20. Superoxide anion production from murine peritoneal macrophage after in vivo activation with poly(VAd) encapsulated in the mannan derivation-coated LUV. — A — Po-ly(VAd)/LUV, - - free poly(VAd), — A — empty LUV without poly(VAd)...

Ph mannan derivatives have been partially characterized and have been... 

A technique which employs substrate affinity chromatography on D-gluco-D-mannan or D-mannan derivatives of agarose has been developed for the purification of legume seed -D-mannanases. Via this technique, j8-d-mannanase B has been purified from lucerne seeds. 

Figure 3 The chemical structure of the coated mannan derivative.

During the past decade, MALDI-TOF MS has proven to be an effective tool for the analysis of oligo- and polymeric mannoglucans (for extensive reviews see [222,223]). SEC/MALDI mass spectrometry was employed in the analysis of hemicelluloses isolated by microwave heat-fractionation from spruce and aspen wood [94]. These methods allowed the separation and characterization of the oligo- and polysaccharide fractions derived from the xylan and mannan components of both woods [224]. 

Benzyl derivatives of (1 6)-a-D-glucan, (l->6)-a-D-mannan, and (l-> 6)-a-D-galactan have been studied in 1,4-dioxane. These derivatives have complex and interesting c.d. spectra due to the ww transition of the chromophore with resolved vibrational structure. However, a conformational interpretation of these interesting spectra is not possible at this time. 

Codium cell-walls contain no crystalline mannan unless they are treated with boiling water. An orthorhombic unit-cell with a = 7.21 A (721 pm), b(fiber axis) = 10.27 A (1.027 nm), and c = 8.82 A (882 pm) was derived. Cell-wall material treated with 12-14% potassium hydroxide gave a different diagram, corresponding to mannan II. In a water-saturated atmosphere, the unit cell for mannan II is monoclinic, with a = 18.8 A (1.88 nm), fo(fiber axis) = 10.2 A (1.02 nm), c = 18.7 A (1.87 nm), and R = 57.5°. 

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