Mammalian cells, physiological activities

This results in the extrusion of three positive charges for every two that enter the cell, resulting in a transmembrane potential of 50-70 mV, and has enormous physiological significance. More than one-third of the ATP utilized by resting mammalian cells is used to maintain the intracellular Na+-K+ gradient (in nerve cells this can rise up to 70%), which controls cell volume, allows neurons and muscle cells to be electrically excitable, and also drives the active transport of sugars and amino acids (see later). 

Mammalian cell lines are the ideal model for studying an effect on the current underlying fKii they allow to use physiological temperatures (37 °C) for the human species HER 293, CHO or L cells expressing hERG channels may be used as a primary test to study the pharmacological activity of a compound... 

Drury AN, Szent-Gyorgyi A (1929) The physiological activity of adenine compounds with especial reference to their action upon the mammalian heart. J Physiol 68(3) 213—237 Englert M, Quitterer U, Klotz K-N (2002) Effector coupling of stably transfected human A3 adenosine receptors in CHO cells. Biochem Pharmacol 64(l) 69-73 Fossetta J, Jackson J, Deno G, Fan X, Du XK, Bober L, Soude-Bermejo A, de Bouteiller O, Caux C, Lunn C, Lundell D, Palmer RK (2003) Pharmacological analysis of calcium responses mediated by the human A, adenosine receptor in monocyte-derived dendritic cells and recombinant cells. Mol Pharmacol 63(2) 342-350... 

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