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MALDI-IMS

TABLE 21.1 Three Major Matrices Used for MALDI-IMS... [Pg.376]

Reliability of MALDI-IMS Images for Drugs in Tissue Sections 371... [Pg.359]

Figure 12.2. Diagram for automated MALDI-IMS system used for direct analysis of pharmaceuticals in tissues. Figure 12.2. Diagram for automated MALDI-IMS system used for direct analysis of pharmaceuticals in tissues.
For imaging of low-molecular-weight compounds using MALDI-IMS, MS/MS instruments are the platforms of choice since regular organic matrices for MALDI are likely to result in background matrix ions that appear at every mass in the low-m/z... [Pg.367]

RELIABILITY OF MALDI-IMS IMAGES FOR DRUGS IN TISSUE SECTIONS 371... [Pg.371]

In a separate experiment, two regions of rat brain tissue sections that contained different levels of clozapine based on the MALDI-IMS results were isolated. The isolated tissue sections were transferred into plastic test tubes and mixed with 300 p,L of 95 5 methanol-water solution for protein precipitation. After centrifugation, a 10-p.L aliquot of the supernatant from each sample was injected for HPLC-MS/MS analysis. The HPLC was operated under a fast gradient condition using a cyano... [Pg.372]

MALDI-IMS has been employed extensively to investigate the distribution of either commercially available or prospective drugs in tissues. Troendle et al. (1999) reported using MALDI in conjunction with a quadrupole ion trap equipped with a laser microprobe to detect the dmg paclitaxel [molecular weight (MW) 853] from rat liver and human ovarian tumor tissue. The liver tissue was incubated with a solution of paclitaxel, while the ovarian tumor tissue was from an animal dosed with paclitaxel in vivo. The concentration of drug was approximately 50mg/kg in each tissue of interest. In both cases, no localization of the dmg was observed. [Pg.374]

This same principle has been further adapted to determine the spatial distribution of both drugs and metabolites in sagittal tissue sections of whole rat with MALDI-IMS (Khatib-Shahidi et al., 2005, 2006). Here the test model compounds (olanzapine, m/z 313 —> 256) propranolol, m/z 260 155) and spiperone, m/z... [Pg.376]

Figure 12.11. (a) Optical image of mouse whole-body section and (b) ion image of SCH 206272 in mouse whole-body section using MALDI-IMS. [Pg.377]

Use of Matrix Assisted Laser Desorption/ Ionization Imaging Mass Spectrometry (MALDI-IMS) in the Development of Novel Small Molecule Drugs... [Pg.399]

The matrix selection and deposition for MALDI-IMS is another crucial step in the sample preparation protocol. The matrix, solvent, concentration of matrix, and the protocol of coating matrix have to be optimized first and then used for the real samples detection. [Pg.406]

See other pages where MALDI-IMS is mentioned: [Pg.375]    [Pg.359]    [Pg.359]    [Pg.359]    [Pg.361]    [Pg.361]    [Pg.361]    [Pg.361]    [Pg.361]    [Pg.363]    [Pg.364]    [Pg.365]    [Pg.365]    [Pg.366]    [Pg.367]    [Pg.367]    [Pg.369]    [Pg.370]    [Pg.371]    [Pg.371]    [Pg.372]    [Pg.373]    [Pg.374]    [Pg.374]    [Pg.374]    [Pg.374]    [Pg.375]    [Pg.376]    [Pg.376]    [Pg.376]    [Pg.377]    [Pg.377]    [Pg.377]    [Pg.378]    [Pg.378]    [Pg.399]    [Pg.400]   


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